of temperature regimes, seed priming and priming duration on germination and
seedling growth on American cotton
Singh1*, N. Gupta2 and M. Dhingra3
Agricultural University Regional Research Station, Faridkot - 151 203, India
Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana - 141
3Department of Soil
Science, Punjab Agricultural University, Ludhiana - 141 004, India
Author E-mail: firstname.lastname@example.org
Bt cotton hybrid
Paper received : 30.08.2016
Revised received : 05.01.2017
Re-revised received :
11.05.2017 Accepted : 24.06.2017???????? ??
Aim: High temperature
during sowing of cotton results in poor emergence and high seedling
mortality, thus leading to poor crop stand and reduced yield. Improvement in
germination by seed priming may combat this problem as this physiological
technique results in faster and synchronous seed germination. Hence, in
vitro studies were conducted to evaluate the effect of different
temperature regimes, seed priming and also duration of priming on germination
and seedling growth to determine and exploit their usefulness in improving
cotton seed germination for better seedling establishment.
of Bt cotton hybrid (Ankur 3028 BGII) were primed separately with water and
KNO3 at different concentrations (2.5, 5.0, 7.5 & 10.0%) for
2, 4 and 6 hrs. Non-primed seeds and hydro primed seeds for 4 hrs were
considered as control. Seeds for each set of treatment were placed on moistened
filter paper in petri dish. Each treatment was then divided into two sub-sets
for assessment of seedling response at two temperature regimes i.e.,
at 25?C and at ambient temperature of 32-38.5?C. After
7 days, final germination percentage, root length, shoot length and fresh
weight of seedling were measured at both temperature regimes. Germination
count was recorded on daily basis and was used to calculate speed of
seeds exhibited an increase in germination by 43% and 34% than control
(non-primed seeds) at ambient temperature (32-38.5?C) and 25?C respectively.
Germination speed also improved by 61% and 67% respectively at ambient
temperature and 25?C over control. Hydropriming resulted in highest
germination (75.5 and 79.2%), germination speed (5.13 and 6.63 days), root
length (4.68 and 6.30 cm) and shoot length (2.59 and 3.40 cm) than control at
25?C and ambient temperature respectively. Contrarily, increase in KNO3
concentration caused deleterious effects on root and shoot length. ????
seed germination was higher at ambient temperature (32?-38.5?C)
than 25?C and priming improved germination potential of seeds.
Hydropriming was more effective than KNO3 for enhancing
germination percentage, germination speed and seedling growth at both
temperature regimes. Studies concluded that hydro priming of cotton seeds
improved germination, a key factor for better crop stand having positive
effect on crop survival, and consequently improved cotton productivity.
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