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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP
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    Abstract - Issue Nov 2017, 38 (6)                                     Back


nstantaneous and historical temperature effects on a-pinene

Host induced genetic variation in legume pod borer, Maruca vitrata

 

S. Sambathkumar1*, C. Durairaj1, S. Mohankumar2, B. Preetha2, R. Aravintharaj2, N. Ganapathy1 and R. Surendran2

1Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore- 641 003 India

2Department of Plant Biotechnology, Tamil Nadu Agricultural University, Coimbatore- 641 003 India

*Corresponding Author E-mail: sambathagritech@gmail.com

 

 

 

Key words

Cluster analysis

Genetic variation

Maruca vitrata

Polymorphism

Similarity index

 

 

 

Publication Data

Paper received : 02.08.2016???????

Revised received : 17.12.2016???

Re-revised received : 20.02.2017

Accepted : 10.05.2017???????????

 

Abstract

Aim: The legume pod borer, Maruca vitrata Fab. is one of the major devastating threats to legume production in all pulse producing countries across the globe. However, it is necessary to acknowledge the role of host plants on M. vitrata.

 

Methodology: In the present study, the influence of major pulse hosts such as pigeonpea, greengram, blackgram, cowpea and lablab on M. vitrata was studied using RAPD, SSR, COI and Wolbachia specific primers.? ??

 

Results: The results clearly revealed that RAPD primers produced 422 (100.0 % polymorphism) polymorphic alleles, whereas SSR primers produced 95 polymorphic alleles with the mean number of alleles detected / primer ranging from 9 to 16 and this showed the existence of wide genetic diversity. Dendrograms constructed using similarity index values for RAPD and SSR primers grouped the larval samples from above pulses into 8 and 6 clusters respectively. Larvae of M. vitrata also yielded specific fragments with mitochondrial COI primer (~800 bp). Additionally, presence of Wolbachia was also confirmed with specific primers.? ????

 

Interpretation: The present study clearly indicated the existence of close proximity in the larval population of cowpea and lablab than other hosts.

 

 

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