Journal of Environmental Biology

Detection of endospore producing Bacillus species from commercial probiotics and their preliminary microbiological characterization

J. Jezewska-Frackowiak¹, K. Seroczynska², J. Banaszczyk³, D. Wozniak⁴, M. Skowron⁵, A. Ozog⁶,

A. Zylicz-Stachula⁷, T. Ossowski⁸ and P. M. Skowron⁹*

^1,6,7,9Department of Molecular Biotechnology, Faculty of Chemistry, University of Gdansk, 80-308, Gdańsk, Poland

^2,3,4GRUPA INCO S.A., 00-519, Warszawa, Poland

⁵BioVentures Institute Ltd., 60-141, Poznan, Poland

⁸Department of Analytical Chemistry, Faculty of Chemistry, University of Gdansk, 80-308, Gdańsk, Poland

*Corresponding Author E-mail: piotr.skowron@ug.edu.pl           

Key words

Bacillus sp.

Industrial probiotics

MALDI-TOF bacteria

Microbiological characterization 

Publication Data

Paper received : 07.10.2016?????????????????? Revised received : 28.03.2017????????????????????? Re-revised received : 24.04.2017 Accepted : 24.06.2017   

Abstract

Aim: The main objective of the study was to characterize a mixture of bacterial species, found in commercial probiotic preparation and originally designed for cleaning, biodegradation and wastewater treatment.

Methodology: Lyophilized preparation of environmental strains was microbiologically characterized to determine the growth temperature range, pH resistance and boiling temperature survivability. Gram staining and Wirtz's spores staining were performed for microscopic estimation of cell morphology and sporulation. The MALDI-TOF mass spectrometry method was used to identify bacterial species found in the preparation.           

Results: The composition of probiotic bacterial species, isolated from commercial lyophilized preparation, exhibited wide range of growth temperatures, extreme boiling survivability and wide range of pH survivability. The isolated species belonged to Bacillus genus, Gram positive and sporulating rods. MALDI-TOF bacterial identification method was carried out for detection of four non-pathogenic, environmental, closely related strains: Bacillus subtilis, Bacillus mojavensis, Bacillus vallismortis and Bacillus pumilus.            

Interpretation: The endospore producing environmental Bacillus species were detected in commercial probiotics and preliminary characterised. The results of the present study, point out the possible applications of the described Bacillus sp. mixture in health, food and cleaning technologies, involving high temperatures or high/low pH processes.

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