in seed vigour traits between desi (pigmented) and kabuli (non-pigmented)
ecotypes of chickpea (Cicer arietinum) and its association with field
Lamichaney1*, S. Kudekallu2, U. Kamble2, N.
Sarangapany2, P.K. Katiyar1 and A. Bohra1
1Division of Crop
Improvement, ICAR Indian Institute of Pulses research, Kanpur-208 024, India
Institute of Seed Science, Mau-275 103, India
Author E-mail: firstname.lastname@example.org
Paper received : 28.04.2016
Revised received : 07.10.2016
Re-revised received :
Accepted : 26.12.2016
Aim: Pigmented (desi)
and non pigmented (kabuli) cultivars of chickpea are known to differ in seed
vigour. Therefore, the main objective of the study was to understand the
mechanisms for such vigour differences and to identify the important seed
coat and seed related vigour traits that makes the coloured desi seeds more
vigorous then unpigmented kabuli seeds.
two chickpea genotypes differing in seed coat colour were included in the
experiment. Field emergence and electrical conductivity of seed leachate was
used as vigour indicator. Hundred-seed weight, proportion of seed coat,
laboratory germination, electrical conductivity, water imbibition pattern,
tannin, lignin and total phenol content, presence or absence of air space
between seed coat and cotyledon and status of hilum-micropylar region were
studied to understand the mechanism for vigour differences between pigmented
desi and unpigmented kabuli genotypes.
a high laboratory germination (>89%) of all cultivars, unpigmented kabuli
genotypes recorded low (39-69%) FE then pigmented desi genotypes (64-87%).
Rapid rate of water imbibition (111.86-145.09%), lower proportion of seed
coat (4.76-6.78%), greater electrical conductivity of seed leachate (49-172
µS cm-1 g-1), low content of lignin (0.74-2.41), tannin
(0.18-1.09 µg mg-1) and total phenol (1.66-5.58 µg mg-1)
was associated with low field emergence in unpigmented kabuli types. Besides,
air space between seed coat and cotyledon, open hilum-micropylar region, less
polyphenolic content and low proportion of seed coat potentially describe the
rapid water uptake by unpigmented kabuli genotypes making them vulnerable to
than laboratory germination, electrical conductivity may be used as an
indicator for determining field emergence in chickpea. Screening/ developing
unpigmented kabuli genotypes with seeds having lower rate of water imbibition
could be a promising way to enable seed vigour improvement in chickpea.