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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue Sep 2017, 38 (5)                                     Back

nstantaneous and historical temperature effects on a-pinene

Ultrastructural studies of Puntius shalynius an endemic

and threatened hill stream fish


M. Manorama* and S. N. Ramanujam

Fish Biology Laboratory, Department of Zoology, School of Life Sciences, North Eastern Hill University, Shillong - 793 022, India

*Corresponding Author E-mail:




Key words

Electron microscopy,              


Puntius shalynius



Publication Data

Paper received : 06.07.2016    

Revised received : 15.11.2016      

Re-revised received : 20.01.2017 Accepted : 16.02.2017



Aim: Shalynai fish, Puntius shalynius is an inhabitant of freshwater, endemic and threatened hill stream fish of North-East India. The present work aimed to study the ultrastructural and functional characteristics of P. shalynius intestine, as addition to studies on nutritional requirement. This fish is considered an important species for ornamental exploitation.


Methodology: A total of five samples were collected from Umiam river, Meghalaya and transported to the laboratory for Transmission Electron Microscopy. Samples from intestine were cut into small pieces, fixed in Karnovsky's fixative, washed in 0.1M sodium cacodylate buffer and examined under an electron microscope. 


Results: The electron microscopy of the anterior intestine from five individuals indicated that the enterocytes were elongated resting on basement membrane and covered apically with uniform microvilli. The apical cytoplasm, just below the microvilli, had no cellular organelles and formed the terminal web. Numerous mitochondria were seen along with other cell organelles such as ribosomes and nucleus. Cellular ultrastructural features were associated with digestive functions.       


Interpretation: The ultrastructural characteristics of P. shalynius intestine showed that a functional specialization exist in this fish also like other teleostean species, a common approach for active digestion in the lack of definite enzymes. The existence of microvilli seems to be important for absorptive function of the cells and enterocytes seems to function primarily in lipid absorption.



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