JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

About Journal
    Editor in Chief
    Editorial Board
    Reviewer Panel
    Publication Policies
    Guidelines for Editors
    Guidelines for Reviewers
    Abstracting and Indexing
    Subscription and Payments
    Contact Journal
Read Journal
    Current Issue
    Journal Archives
For Authors
    Guidelines for Authors
    Terms and Conditions
    Fees and Payments
    Track Paper Status
    JEB Awards

Google Search the Journal web-site:

    Abstract - Issue Sep 2017, 38 (5)                                     Back

nstantaneous and historical temperature effects on a-pinene

In-vitro studies on antioxidant, anti-melanogenic and anti-wrinkle effects of medicinal plant Glycyrrhiza uralensis


A.R. Ryu1, M.J. Kang1 and M.Y. Lee1,2*

1Department of Medical Science, Soonchunhyang University, Asan,Chungnam, 31538, Korea

2Department of Medical Biotechnology, Soonchunhyang University,Asan, Chungnam, 31538, Korea

*Corresponding Author E-mail:




Key words


Antioxidant effect,

Anti-wrinkle effect,

Glycyrrhiza uralensis



Publication Data

Paper received : 23.07.2016

Revised received : 19.01.2017

Accepted : 23.03.2017



Aim: The aim of the present study was to investigate the antioxidant, anti-melanogenic and anti-wrinkle activity of Glycyrrhiza uralensis (G. uralensis) for cosmeceutical application.


Methodology: The free radical scavenging activity of 1,3-butylene glycol (1,3-BG) extract of G. uralensis was analyzed using 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC) assays. The anti-melanogenesis and anti-wrinkle effects of G. uralensis extract were also analyzed by tyrosinase, zymography and collagen assays.


Results: The EC50 of 1,3-BG extract of G. uralensis, determined by DPPH and ABTS assays, was 0.59% and 1.17%, respectively.The ORAC value of the extract was 41.56 μM trolox equivalent. The G. uralensis extract inhibited in vitro mushroom tyrosinase activity with an IC50 of 0.6%, and it also reduced melanin contents in mouse melanoma cells. In addition, the G. uralensis extract notably inhibited matrix metalloproteinase-2 activity via zymography. Approximately, 60% collagen production enhancement was observed upon treatment with the extract, suggesting its anti-wrinkle potential. ?????


Interpretation: The 1,3-BG extract of G. uralensis may be utilized as a cosmeceutical ingredient, offering anti-oxidant, brightening and anti-wrinkle effects.



Copyright ? 2017 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).