Journal of Environmental Biology

In-vitro studies on antioxidant, anti-melanogenic and anti-wrinkle effects of medicinal plant Glycyrrhiza uralensis

A.R. Ryu¹, M.J. Kang¹ and M.Y. Lee^1,2*

¹Department of Medical Science, Soonchunhyang University, Asan,Chungnam, 31538, Korea

²Department of Medical Biotechnology, Soonchunhyang University,Asan, Chungnam, 31538, Korea

*Corresponding Author E-mail: miyoung@sch.ac.kr

Key words

Anti-melanogenesis,

Antioxidant effect,

Anti-wrinkle effect,

Glycyrrhiza uralensis

Publication Data

Paper received : 23.07.2016

Revised received : 19.01.2017

Accepted : 23.03.2017

Abstract

Aim: The aim of the present study was to investigate the antioxidant, anti-melanogenic and anti-wrinkle activity of Glycyrrhiza uralensis (G. uralensis) for cosmeceutical application.

Methodology: The free radical scavenging activity of 1,3-butylene glycol (1,3-BG) extract of G. uralensis was analyzed using 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC) assays. The anti-melanogenesis and anti-wrinkle effects of G. uralensis extract were also analyzed by tyrosinase, zymography and collagen assays.

Results: The EC₅₀ of 1,3-BG extract of G. uralensis, determined by DPPH and ABTS assays, was 0.59% and 1.17%, respectively.The ORAC value of the extract was 41.56 μM trolox equivalent. The G. uralensis extract inhibited in vitro mushroom tyrosinase activity with an IC₅₀ of 0.6%, and it also reduced melanin contents in mouse melanoma cells. In addition, the G. uralensis extract notably inhibited matrix metalloproteinase-2 activity via zymography. Approximately, 60% collagen production enhancement was observed upon treatment with the extract, suggesting its anti-wrinkle potential. ?????

Interpretation: The 1,3-BG extract of G. uralensis may be utilized as a cosmeceutical ingredient, offering anti-oxidant, brightening and anti-wrinkle effects.

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