of polycyclic aromatic hydrocarbons by
species isolated from ecological sources
Al-Hadithi1, E.A. Al-Razzaq2 and G.F. Fadhil3*
Basic Pharmaceutical Sciences, University of Isra, College of Pharmacy,
Amman, 11622, Jordan
Biology, College of Science, University of Basrah, Basrah, 61009, Iraq
Chemistry, College of Science, University of Duhok, Duhok, 1048BD, Iraq
Author E-mail: email@example.com
Paper received : 20.07.2016
Revised received : 21.12.2016
Re-revised received :
Accepted : 09.03.2017?
Aim: The objective of
the present study was to isolate Acinetobacter from ecological sources
in Basrah city, the Iraq?s main port located on the Shatt al-Arab, to
identify recovered species and to investigate their capability for
bioremediation and degradation of Polycyclic aromatic hydrocarbons (PAH).
hundred and five samples were collected from Municipal drinking water, soil
and surface water of Shat Al-Arab waterway area / Basrah / Iraq, polluted
with diesel oil. Two species of Acinetobacter were isolated and
identified according to diagnostic tests. Bioremediation of PAHs was
investigated by incubating? 5 x108 bacterial cell ml-1 of either
two species in a mineral medium and examined after two weeks, one month and
two months adopting gravimetric and spectrophotometric methods. The bacterial
density of each species was determined after each incubation period.
Acinetobacter isolates were obtained in a frequency distribution of
9.2523%. Isolates were isolated from three sources and? identified as A.
lwoffii (8%), however, A. calocoacetcus (2%) was isolated from
soil only. Starting with 5x108 bacterial cell ml-1 of
A. calocoacetcus; the count was increased to 8.05x1011cell ml-1
after two weeks then decreased to 6.3x105 cell ml-1
after one month and became 0.2x102 bacterial cell ml-1
after two months. Accordingly, the weight of PAHs decreased from 0.1 g to
0.053, 0.013 then to 0.001 g 250 ml-1 respectively. The same
sequence of events was demonstrated by A. lwoffii, though with a
higher rate of degradation confirming its higher potency for bioremediation. ?????
to bioremediation, the weight of PAH decreased with a concomitant decrease in
the growth and activity of either species of Acinetobacter. Hence, it
is a promising alternative for cleaning oil spills and reducing the
concentration and toxicity of pollutants.
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