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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue Sep 2017, 38 (5)                                     Back

nstantaneous and historical temperature effects on a-pinene

Bioremediation of polycyclic aromatic hydrocarbons by

Acinetobacter species isolated from ecological sources


H.T. Al-Hadithi1, E.A. Al-Razzaq2 and G.F. Fadhil3*

1Department of Basic Pharmaceutical Sciences, University of Isra, College of Pharmacy, Amman, 11622, Jordan

2Department of Biology, College of Science, University of Basrah, Basrah, 61009, Iraq

3Department of Chemistry, College of Science, University of Duhok, Duhok, 1048BD, Iraq

*Corresponding Author E-mail:




Key words

Acinetobacter species,


Polycyclic aromatic hydrocarbons 



Publication Data

Paper received : 20.07.2016

Revised received : 21.12.2016

Re-revised received : 01.03.2017

Accepted : 09.03.2017?



Aim: The objective of the present study was to isolate Acinetobacter from ecological sources in Basrah city, the Iraq?s main port located on the Shatt al-Arab, to identify recovered species and to investigate their capability for bioremediation and degradation of Polycyclic aromatic hydrocarbons (PAH).


Methodology: One hundred and five samples were collected from Municipal drinking water, soil and surface water of Shat Al-Arab waterway area / Basrah / Iraq, polluted with diesel oil. Two species of Acinetobacter were isolated and identified according to diagnostic tests. Bioremediation of PAHs was investigated by incubating? 5 x108 bacterial cell ml-1 of either two species in a mineral medium and examined after two weeks, one month and two months adopting gravimetric and spectrophotometric methods. The bacterial density of each species was determined after each incubation period.


Results: Ten Acinetobacter isolates were obtained in a frequency distribution of 9.2523%. Isolates were isolated from three sources and? identified as A. lwoffii (8%), however, A. calocoacetcus (2%) was isolated from soil only. Starting with 5x108 bacterial cell ml-1 of A. calocoacetcus; the count was increased to 8.05x1011cell ml-1 after two weeks then decreased to 6.3x105 cell ml-1 after one month and became 0.2x102 bacterial cell ml-1 after two months. Accordingly, the weight of PAHs decreased from 0.1 g to 0.053, 0.013 then to 0.001 g 250 ml-1 respectively. The same sequence of events was demonstrated by A. lwoffii, though with a higher rate of degradation confirming its higher potency for bioremediation. ?????


Interpretation: Owing to bioremediation, the weight of PAH decreased with a concomitant decrease in the growth and activity of either species of Acinetobacter. Hence, it is a promising alternative for cleaning oil spills and reducing the concentration and toxicity of pollutants.



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