diversity of sugar beet genotypes evaluated
microsatellite DNA markers
Srivastava*, A. D. Pathak, R. Kumar and B.B. Joshi
Division of Crop
Improvement, ICAR-Indian Institute of Sugarcane Research, Lucknow-226 002,
Author E-mail: email@example.com
Paper received : 19.08.2015
Revised received :
Re-revised received :
Accepted : 15.02.2017
Aim: Identification of
diverse sugar beet (Beta vulgaris L.) genotypes is essential for using
them as parents in sugar beet improvement programme of India. Hence, genetic
variation and diversity of elite sugar beet germplasm collection was analysed
using microsatellite markers.
DNA of thirteen genotypes was amplified using 14 microsatellite primers
containing dinucleotide to compound repeat motifs. Amplified bands were
scored on gel, transformed into a binary character matrix and analyzed
through NTSYS software for clustering in heterotic groups.
total of 243 amplicons were resolved and grouped into 88 alleles of distinct
molecular weight ranging from 124 to 1222 bp with an average of 17.36
amplicons/primer, 4 to10 alleles/SSR locus and moderate to high PIC ranging
from 0.625-0.851. Similarity coefficients (SM) based on the presence/absence
of alleles ranged from 0.47 to 0.89 (mean value of 0.65). UPGMA dendrogram
based on SM, grouped these genotypes in two major taxonomical groups with
five clusters having one, four, three, three and two genotypes each.
Clustering pattern of UPGMA matched with the 2D and 3D scatter plots of MDS
and PCA. The highly significant cophenetic coefficient of r = 0.96 proved
that these SSR markers efficiently analyzed the genetic relationships of
sugar beet genotypes.
markers can be used as a potential cost effective method for exploring
molecular genetic diversity in sugar beet in order to obtain new genetic
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