and identification of oleaginous moulds for lipid production
Sheerin Banu1*, Ramprasad Kuncham2, M.A. Azeem3
and M.R. Bharath1
Development Centre, Bharathiar University, Coimbatore-641 046, India
India Pvt. Ltd., Whitefield, Bengaluru-560 048, India
Pharmacognosy, Al-Ameen College of Pharmacy, Bangalore-560 027, India
received : 18.03.2017??????
received : 27.03.2017???
received : 08.04.2017
Accepted : 02.05.2017
Aim: The present study
was carried out to isolate the moulds from garden soils of Lalbagh Botanical
Garden and Cubbon Park Bangalore City, India and screen them for their
types of moulds were isolated randomly from garden soils and screened for
their lipid production. Twenty five moulds were screened for their abilities
to produce lipids. Potential lipid producers were identified by cultural
method and confirmed by ITS region gene Sanger DNA sequencing. The fatty acid
profile of two mould isolates was identified using GC-FID.
two isolates such as GS7 and GS12 were found to be promising lipid producers
among twenty five screened moulds, and maximum lipid contents found in GS7
and GS12 were 22 and 24.3%, respectively. The mould isolates were identified
as Penicillium citrinum (GS7) and Trichoderma asperellum (GS12)
using colony characteristics and Sanger Sequencing. Consensus gene sequences
were submitted to NCBI genbank and Accession numbers (KX865284 and KY623504)
were obtained. The fatty acid profile of Pencillium citrinum showed
56.85% of saturated fatty acid (SFA), 32.20% of monounsaturated fatty acids
(MUFA) and 10.95% polyunsaturated fatty acids (PUFA), while Trichoderma
asperellum showed 47.73% ofsaturated fatty acid (SFA), 25.12% of
monounsaturated fatty acids (MUFA) and 27.09% polyunsaturated fatty acids
(Penicillium citrinum) and GS12 (Trichoderma asperellum)
described in the study were identified as promising lipid producers. This
study would help in better selection of moulds for producing lipids which are
suitable for biodiesel production.
? 2017 Triveni Enterprises. All rights reserved. No part of the Journal can
be reproduced in any form without prior permission. Responsibility
regarding the authenticity of the data, and the acceptability of the
conclusions enforced or derived, rest completely with the author(s).