of culturable detrimental biofilm-forming bacteria
wastewater treatment system
Güvensen*, Z. Zorlu and B. Çöl
Department, Faculty of Sciences, Muğla Sıtkı Koçman
University, Muğla, 48170, Turkey
Author E-mail: firstname.lastname@example.org
Wastewater treatment system
received : 29.01.2016
received : 27.06.2016
received : 27.10.2016
Accepted : 22.12.2016
Aim: Biofilms are
sessile communities of cells attached to a surface or to each other, usually
embedded in polymeric substances produced by the bacteria. The community
structure of bacteria in the biofilm formed in industrial systems is the
basic knowledge to understand the complexity and mechanisms of biocorrosion.
The major objective of the present study was to find out the bacterial
biodiversity of detrimental biofilm masses from an industrial water treatment
system in Köyceğiz-Muğla, Turkey.
biofilm sample was collected from the waste water treatment plant of
Köyceğiz-Muğla, Turkey. For phenotypic identification of bacterial
isolates from sample, basic morphological and biochemical tests were
performed, as reported in Bergey's Manual of Systematic Bacteriology. These
bacteria were identified based on 16S rRNA gene sequence analysis for
phylogenetic characterization. Additionally, the biofilm-forming ability of
all the strains were evaluated in the study by modified biofilm ring test and
by semi-quantitative method (microtiter plate assay). Microtiter plate assay
is based on the colorimetric measure of a dye such as crystal violet
incorporated by sessile cells.
identification tests indicated that nineteen strains belonged to four
different groups. Among four firmicutes (74.0%) were found to be dominant
phylum. The majority of isolated strains were affiliated to family
Bacillaceae (69.5%). Other strains from the biofilm were affiliated to
Enterobacteriaceae (10.5%), Comamonadaceae (5.0%), Microbacteriaceae (5.0%)
and Nocardiaceae (5.0%), family respectively in Group Gammaproteobacteria
(10.5%), Betaproteobacteria (5.0%) and phylum Actinobacteria (10.5%).
Generally, all the nineteen strains were able to form biofilm strongly.
may be a source of recalcitrant and xenobiotic contaminations, causing
environmental detriment and possible source of public health problems such as
outbreaks of waterborne pathogens. The study revealed a high bacterial
diversity in the biofilm.
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