of leaf micro-morphological features with
mosaic disease resistance in pigeonpea
Patil1 ,2*, H.E. Shashidhar2, M. Byregowda2,
G.A.M. Reena2, T.H. Ashok2, H.V.V. Swamy2, H.K.
Ramappa2 and J. Babu2?
1Division of Plant
Biotechnology, Indian Institute of Pulses Research, Kanpur-208 024, India
Plant Biotechnology, AICRP on Pigeonpea, University of Agricultural Sciences,
GKVK, Bengaluru -560 065, India
Author E-mail: firstname.lastname@example.org
Sterility mosaic disease
received : 18.02.2016
received : 25.07.2016
received : 23.11.2016
Accepted : 01.12.2016
Aim: Sterility mosaic
disease (SMD) is one of the major disease in pigeonpea, which causes heavy
yield losses upto 95 % in India. The disease is caused by pigeonpea sterility
mosaic virus (PPSMV) and transmitted by eriophyid mite (Aceria cajani).
The occurrence of five different isolates of virus were reported, which are
transmitted by single biotype of mite. Therefore, the present study was
carried out to identify important leaf structural features of pigeonpea that
impart resistance to mite vector, there by helping in broad based resistance
breeding against SMD pathogens.
three extreme pigeonpea genotypes showing resistance (9) and susceptible (14)
reaction to SMD were selected from seventy genotypes, which were screened for
Bengaluru isolate of SMD. Data were recorded on twelve important leaf
micro-morphological traits including trichome number and length on the upper
and lower surface of leaf in cultivars and wild belonging to resistance and
susceptible groups. The promising leaf features which were significantly
differing, correlating and with higher discriminant function were identified
from the resistant and susceptible groups of cultivars, wild and across using
three statistical methods such as paired t-test, correlation and discriminant
cultivars, specific leaf area (SLA) and specific leaf weight (SLW), in wild
trichome number on upper surface (TNUS) and lower surface (TNLS) of leaf, and
for across genotypes trichome length on lower surface of leaf (TLLS) and SLA
were found most informative. Association of these traits across the genotypes
revealed that TNUS, TNLS, TLLS and SLW were significantly and negatively
associated with percent disease incidence (PDI). Considering these traits,
discriminant functions were developed to identify resistant genotypes.
Scatter plots based on SLA and SLW classified pigeonpea cultivars with 100%
accuracy, TNLS and TNUS classified wild genotypes with 83.3% accuracy, TLLS
and SLA classified both cultivar and wild with 76.6 % accuracy in relation to
leaf micro-morphological features such as TNUS, TNLS, TLLS, SLA and SLW were
found significantly differing, correlating and with higher discriminant
function in relation to SMD disease response. These traits are possibly
involved in bringing vector mediated resistance to SMD.?????? ??????????