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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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        Abstract - Issue Jul 2017, 38 (4)                                                                                                             Back

nstantaneous and historical temperature effects on a-pinene

In situ nutrient-spiking bioassays for determining phosphorus and

light limitation in a wetland ecosystem


Woon-Ki Moon, Ji-Woong Choi, Sang-Jae Lee and Kwang-Guk An*

Department of Biological Sciences, College of Biological Sciences and Biotechnology, Chungnam National University, Daejeon - 305 764, Korea

*Corresponding Author E-mail:




Key words

Algal growth,

Nutrient spiking bioassay,


Suspended solids,

Wetland ecosystem



Publication Data

Paper received : 23.11.2015

Revised received : 15.03.2016

Re-revised received : 10.10.2016

Accepted : 09.11.2016



Aim: The objective of the present study was to determine the factors that regulate the response of algal growth in wetland ecosystem by in situ nutrient-spiking bioassays (NSBs).


Methodology: The NSBs tested algal responses as a ratio of final (Chlf) to initial Chl (Chli) in the control (no nutrient addition) and several treatments of phosphorus (P), nitrogen (NH4-N, NO3-N), and NO3-N+P under various environmental conditions of inorganic suspended solids (ISS) and free-floating plants (FfP).


Results: Experiments of in situ NSBs with low inorganic solids showed that the response of P treatments were significantly (p< 0.05) greater than the controls and the treatments of NH4-N or NO3-N. Regression analysis of Chlf : Chli ratios against log10-transformed TP in the cubitainers, thus showed that in situ algal response in P treatments was directly determined (R2 = 0.965, F = 40.049, p< 0.001) by concentrations of spiked TP. In contrast, the in situ NSBs with high ISS (> 15 mg l-1) or high FfP cover (> 95%) showed that the response in treatments of N, P or P+NO3-N had no significant (p> 0.05) difference with the controls.


Interpretation: Light limitation dominated the system, thus the ratio of Chlf : Chli had no relation (p> 0.10) with the spiked TP. Overall, the experiments of in situ NSBs suggest that high P-inputs from the watershed increased algal growth, but dense FfP or high inorganic solids suppressed eutrophication in the wetland.



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