of extended-spectrum β-lactamase producing Escherichia coli
in seafood? from the retail fishery outlets of Veraval, Gujarat, India
Sivaraman1*, M.M. Prasad2,? A.K. Jha1, S.
Visnuvinayagam3, V. Renuka1, S. Remya1, Y.
Kriplani1 and D. Vanik1
Research Centre of Central Institute of Fisheries Technology, Veraval- 362
Fermentation and Biotechnology Division, ICAR- Centre of Institute of
Fisheries Technology, Cochin- 682 029, India
Research Centre of Institute of Fisheries Technology, Navi Mumbai- 400 703,
Author E-mail: email@example.com
Multi drug resistance,
received : 26.05.2016
received : 30.07.2016
received : 09.11.2016
Accepted : 16.12.2016
Aim: Profound and
unregulated utilization of beta-lactam antibiotics for the treatment of
various infections has lead to various bacteria being resistant against these
antibiotics. E. coli is well known to produce extended, spectrum β-
lactamase (ESBL) type of β- lactamase, which has the ability to
hydrolyse the entire group of β- lactam antibiotics. Currently,
reports on the occurrence of ESBL producing E. coli in seafood is
meagre. Therefore, the objective of this study was to monitor the prevalence
of multiple drug resistant (MDR) and ESBL producing E. coli in seafood
in Veraval region of Gujarat.
and isolation of E. coli was carried out using ISO, 9308-1 method with
minor modification. A 0.5 ml of serially diluted fish sample was spread on
Tergitol-7 media (Oxoid, CM0793) and incubated at 35oC for 24 hrs.
Typical flat, dry and yellow colonies with or without red tinge were further streaked
on EMB plates and then subjected to IMViC and 20E BioMerieux identification
kit. All the confirmed E. coli isolates were subjected to antimicrobial
susceptibility testing, and Minimum Inhibitory Concentration (MIC) was
total of 28 number of E. coli (approx 12%) isolates were identified
from 238 fish samples. All these isolates were subjected to antimicrobial
susceptibility testing and a higher rate of resistance was found with
ampicillin (39.29%), trimethoprim/ sulfamethoxizole (32.14%), ciprofloxacin
(28.57%) cefepime (17.86%) and cefuroxime (17.86%). All these isolates were
sensitive to carbapenems and beta-lactam, beta-lactam inhibitors and
aminoglucosides. Out of total 28, E. coli isolated (15.71%) eleven
isolates were found to be multi-drug resistant and four (14.29%) isolates
were ESBL producers
study confirms the presence of hazardous MDR and ESBL producing E. coli
in fish and fishery products in Veraval, Gujarat.
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