Journal of Environmental Biology

Effect of polyherbal extract on tca cycle enzymes and heart tissue histology in isoproterenol induced myocardial rats

B. Ragavan* and S. Monisha

Department of Biochemistry, PSG college of Arts and Science, Coimbatore-641 014, India

*Corresponding Author E-mail: moni1487@gmail.com

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Key words

Histopathology,

Myocardial infarction,

Polyherbal crude extract,

TCA cycle enzymes

Publication Data

Paper received : 08.09.2016

Revised received : 16.11.2016

Re-revised received : 11.01.2017

Accepted : 19.01.2017

Abstract

Aim: Cardiovascular disease has become a universal cause of morbidity and largely contributes to mortality in developed and developing countries. The present study aimed to evaluate the cardioprotective role of polyherbal extract on isoproterenol induced myocardial infarction in Wistar albino rat model.

Methodology: The rats were divided into eight groups each having six animals. Treatment I served as control, Treatment II rats were administered isoproterenol, Treatment III and IV were pretreated with polyherbal extract and received a subcutaneous injection of isoproterenol, Treatment V and VI were pretreated orally with propranolol and received a subcutaneous injection of isoproterenol, Treatment VII and VIII received polyherbal extract  for 30 days. After cervical dislocation, the heart tissues were removed and studied for TCA cycle and respiratory chain enzymes along with histopathological observation.

Results: Isoproterenol induction exhibited significant (p<0.05) reduction in the enzyme activities of mitochondrial TCA cycle. However, there was a significant (p<0.05) increase in polyherbal treated rats of heart tissue homogenate. The effect of oral administration of polyherbal crude extract at 500 mg g^-1 b.wt. was found to have more effect than  250 mg g^-1 b.wt. Similarly, the standard drug propranolol 20 mg g^-1 b.wt. treated rats also exerted an effective impact. The histological sections prepared from isoproterenol hydrochloride treatment alone exhibited various degrees of focal lesions in several sections, consisting of staining and fragmentation of muscle fibers with confluent retrogressive lesions. Animals treated with polyherbal crude extract showed significant improvement in ISO-induced alterations such as vacuolar modifications, edema, capillary dilatation and leukocyte infiltration compared to ISO- administered group.   

Interpretation: Polyherbal crude extract exhibited synergistic cardioprotective action which was revealed by improving the TCA cycle enzyme and protective effect on myocardium when compared to standard drug propranolol. 

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