of polyherbal extract on tca cycle enzymes and heart tissue histology in
isoproterenol induced myocardial rats
Ragavan* and S. Monisha
Biochemistry, PSG college of Arts and Science, Coimbatore-641 014, India
Polyherbal crude extract,
TCA cycle enzymes
received : 08.09.2016
received : 16.11.2016
received : 11.01.2017
Accepted : 19.01.2017
disease has become a universal cause of morbidity and largely contributes to
mortality in developed and developing countries. The present study aimed to evaluate
the cardioprotective role of polyherbal extract on isoproterenol induced
myocardial infarction in Wistar albino rat model.
rats were divided into eight groups each having six animals. Treatment I
served as control, Treatment II rats were administered isoproterenol,
Treatment III and IV were pretreated with polyherbal extract and received a
subcutaneous injection of isoproterenol, Treatment V and VI were pretreated
orally with propranolol and received a subcutaneous injection of isoproterenol,
Treatment VII and VIII received polyherbal extract for 30 days. After
cervical dislocation, the heart tissues were removed and studied for TCA
cycle and respiratory chain enzymes along with histopathological observation.
induction exhibited significant (p<0.05) reduction in the enzyme
activities of mitochondrial TCA cycle. However, there was a significant
(p<0.05) increase in polyherbal treated rats of heart tissue homogenate.
The effect of oral administration of polyherbal crude extract at 500 mg g-1
b.wt. was found to have more effect than 250 mg g-1 b.wt.
Similarly, the standard drug propranolol 20 mg g-1 b.wt. treated
rats also exerted an effective impact. The histological sections prepared
from isoproterenol hydrochloride treatment alone exhibited various degrees of
focal lesions in several sections, consisting of staining and fragmentation
of muscle fibers with confluent retrogressive lesions. Animals treated with
polyherbal crude extract showed significant improvement in ISO-induced
alterations such as vacuolar modifications, edema, capillary dilatation and
leukocyte infiltration compared to ISO- administered group.
crude extract exhibited synergistic cardioprotective action which was
revealed by improving the TCA cycle enzyme and protective effect on
myocardium when compared to standard drug propranolol.
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