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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue May 2017, 38 (3)                                     Back

nstantaneous and historical temperature effects on a-pinene

A study on concentration of total petroleum hydrocarbons in lentic water bodies near oil field areas of upper Assam, India


B. Basumatary1, M. K. Das2*, S. Baishya2 and S. Bordoloi2

1Department of Botany, North Lakhimpur College (Autonomous), Lakhimpur-787 031, India

2Resource Management and Environment Section, Life Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati-781 035, India

*Corresponding Author E-mail:




Key words

Lentic water body,

Oil fields,

Petroleum hydrocarbons




Publication Data

Paper received : 09.09.2015

Revised received : 23.02.2016

Re-revised received : 22.07.2016

Accepted : 13.09.2016



Aim: Contamination of water and soil by crude oil and their accumulation in biota as a result of exploration, production, maintenance, transportation, storage and accidental release, add hazardous chemicals to the ecosystem. To determine the spatial and temporal variations of total petroleum hydrocarbon (TPH) concentrations in water, sediment and plant samples, a study was conducted in ten selected lentic water bodies in and around oil fields, group gathering stations and oil collecting stations of  upper Assam, India.


Methodology: The samples (water, sediment and plant) were collected seasonally during June, 2013 to May, 2014.  Water samples were collected from the surface in amber colored glass bottles. The sediment samples were collected from 15 - 30cm depth below the water surface with soil sampler from three points (1/4, 1/2 and 3/4) across the lentic water bodies in sterile polythene bags. Aquatic plant samples were uprooted, carefully separated from the soil and rinsed. The samples were transported to the laboratory in ice boxes under low temperature conditions and TPH extraction was done by standard methods. The extracted samples from water, sediment and plants were analysed for TPH in Gas Chromatography.


Results: Results showed that both water and sediments contained significant (p < 0.05) concentration of TPH ranging between 89 to 843 mg l-1 and 9843.35 to 59194.48 mg kg-1 respectively. The collected plants also showed the presence of significant (p < 0.05) concentration of TPH in shoot (295 to 872 mg kg-1) and in root (178 to 617 mg kg-1).  


Interpretation: The TPH concentration in water, sediments and plants were relatively higher during the post monsoon season compared to winter and monsoon seasons. The TPH content in plants varied considerably with species. 



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