preliminary study on the antimicrobial activity
Dhevagi1*, A. Brundha2, K. Geetha2 , R. Gobu2
, K.A.A. Manju2 and E. Poorani3
Environmental Sciences, Tamil Nadu Agricultural University, Coimbatore -641 003,
Environmental Sciences, Tamil Nadu Agricultural University, Coimbatore-641
Biotechnology, Government College of Technology, Coimbatore-641 013, India
Author E-mail: firstname.lastname@example.org
received : 09.02.2016
received : 18.06.2016
received : 10.09.2016
Accepted : 09.11.2016
Aim: Marine actinomycetes
are considered as an unexplored source of metabolites with novel properties.
During the past few years, the studies on marine actinomycetes highlighted
their potential as source of numerous antibiotics. An experimental study was
planned to harness the potential of marine actinomycetes as a source of
bioactive compounds to control plant diseases.
samples from Thoothukudi coastal ecosystem were collected and analyzed for
biological properties. The isolates with different morphological
characteristics were selected and about 108 isolates were subjected to cross
streak method of screening against fungal cultures like Pyriculariae
oryzae, Aspergillus niger, Fusarium solani, Trichoderma, and Macrophomina
sp and bacterial strains like Bacillus cereus, Erwinia carotovora,
Serratia sp., Flavobacterium sp and Pseudomonas fluorescens.
Solvent extract of isolates producing significant zone of inhibition were
screened against plant pathogens.
isolates produced an inhibition zone of 2 to > 10mm against the fungal and
bacterial cultures. The isolate TS3 obtained from TS sediments showed more
than 10 mm inhibition zone against Erwinia caratovora, Pyriculariae
oryzae, Aspergillus niger and Trichoderma. The isolates which showed
more than 10 mm antifungal and antibacterial activity were selected for
further screening. Ehtylacetate extract of RPS5 showed an inhibition zone of
9 to 14 mm and 18 to 22 mm, respectively, against bacterial and fungal
pathogens. Similarly, HCS 6 isolates showed an inhibition zone of 9 to 12 mm
against bacterial pathogens and 16 to 22 mm inhibition zone, respectively,
against fungal pathogens.
the environmental impacts of chemical fungicides, control of pathogens through
biological means helps to overcome the worsening problem caused by
chemicals. The solvent extract of RPS 5 and HCS 6 isolates showed significant
reduction in the growth of pathogenic organisms, which paves way for
commercial exploitation of the isolates to control plant pathogens.
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