Journal of Environmental Biology

Studies on the effect of temperature variation on chromosomal changes in buckwheat (Fagopyrum esculentum Moench)

G. Kumar and A. Srivastava*

Department of Botany, University of Allahabad, Allahabad-211 002, India

*Corresponding Author E-mail: srivas.akanksha20@gmail.com ?

Key words

Abnormality percentage,

Active mitotic index,

Chromosomal aberrations,

Fagopyrum esculentum,

Temperature stress

Publication Data

Paper received : 12.08.2015

Revised received : 08.01.2016

Re-revised received : 23.06.2016

Accepted : 13.07.2016

Abstract

Aim: Climatic fluctuations consequent into various kinds of environmental stresses.? Among these, temperature stress is a major concerning factor with respect to plants. The aim of the present investigation was to study the effect of temperature stress on plant mitosis for different durations in the root meristems of Fagopyrum esculentum (buckwheat). Buckwheat is a sensitive plant with response to temperature stress.

Methodology: Exposure of cold and heat stress was given for 3, 5 and 7 hrs to the root meristems of Fagopyrum esculentum, and their effects were studied at cytological level along with various aspects.

Results: Active mitotic index and chromosomal abnormalities were calculated that showed inverse relationship to each other. Different types of abnormalities were observed comprising scattering, c-mitosis, precocious movement, stickiness, unorientation, laggard formation, bridge formation etc., alongwith increasing duration of temperature. ??? 

Interpretation: After calculating active mitotic index (AMI %) and abnormality percentages, it was concluded that heat stress was more effective as it showed various chromosomal anomalies in the root meristems of Fagopyrum esculentum as compared to cold stress. 

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