Journal of Environmental Biology

Genetic diversity and population structure analysis of

Chrysanthemum (Dendranthema grandiflora Tzvelev)

germplasm based on RAPD markers

S. Kumar¹*, M. Kumar¹, H. K. Yadav², S. Sharma³ and S. Kumar¹

¹Department of Horticulture, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut-250 110, India

²Genetics and Molecular Biology Division, CSIR-National Botanical Research Institute, Lucknow-226 001, India

³Department of Crop Science, Institute of Genomic Biology, University of Illinois, Urbana-Champaign-61801, USA

*Corresponding Author E-mail: drskflori@yahoo.com

Key words

Chrysanthemum,

Cluster,

Genetic diversity,

Population structure,

RAPD

Publication Data

Paper received : 04.01.2016

Revised received : 13.03.2016

Re-revised received : 15.07.2016

Accepted : 05.08.2016

Abstract

Aim: The present investigation was carried out to characterize the genetic variation present in a set of 38 Indian chrysanthemum cultivars using RAPD (random amplified polymorphic DNA) markers. Unlike to previous molecular markers based diversity studies, the present study involved population structure analysis which revealed subpopulations and admixtures in set of chrysanthemum cultivars studied.

Methodology: The experimental material of the present investigation comprised of 38 chrysanthemum cultivars collected from different part of India. Total genomic DNA was extracted from fresh and young leaf tissues following CTAB method. Polymorphic information content and resolving power of primers were calculated to select useful primers. Dendrogram and PCoA analysis were carried out to reveal the genetic diversity and relatedness among cultivars. Population structure analysis was carried out to decipher any subpopulations and admixtures in the chrysanthemum germplasm.

Results: A total of 70 clear and scorable bands were produced by 10 RAPD primers. Out of 70 bands, 66 were polymorphic and four were monomorphic. All the 38 cultivars were grouped into 2 sub-population. Mixed population ancestry was observed among the two clusters.    

Interpretation: Sufficient diversity was observed among the germplasm. Results of present study will be helpful for future population and selective breeding studies targeting overall chrysanthemum improvement. Diverse cultivars identified in the present study can be utilized in multi-parent breeding program to develop varieties with desirable traits. 

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