of plant growth promoting rhizobia from root nodule of Mimosa pudica
grown in Assam, India
Singha1, P. B. Mazumder1* and P. Pandey2
Biotechnology, Assam University, Silchar-788 011, India
2Department of Microbiology,
Assam University, Silchar-788 011, India
Author E-mail: email@example.com
received : 08.01.2016
received : 23.05.2016
received : 30.09.2016
Accepted : 06.10.2016
Aim: Rhizobia are
diverse group of soil microorganisms that can form nodule in the roots of
leguminous plants and fixes atmospheric nitrogen. In the present study, an attempt
was made to characterize the plant growth promoting rhizobia from root
nodules of Mimosa pudica grown in Assam.
bacterial isolates were investigated for their phenotypic features. Plant
growth promoting abilities of the isolates were determined by conducting
different tests. Genotypic characterization of the isolates was carried out
by 16S rDNA PCR-RFLP and 16S rDNA gene sequencing. Symbiotic efficiency of
the isolates was determined by nodulation test.
isolates showed similarity in colony morphology, shape and Gram staining, but
showed variation in biochemical features. Most of the isolates produced
indole acetic acid, ammonia and solubilized inorganic phosphate. Dendrogram
based on PCR-RFLP analysis of 16S rDNA gene revealed significant diversity
among the isolates. Phylogenetic analysis of the isolates, based on 16S rDNA
gene sequences, revealed that SMP2, HMP1 and DMP2 isolates were closely
related to Burkholderia mimosarum PAS44, while KMP1 was closely
related to Mesorhizobium loti MAFF303099.
present study revealed that bacterial strains from Burkholderia genera
were the major symbionts of Mimosa pudica grown in Assam. The isolates
obtained in the present study possesed plant growth promoting abilities and
could be used as a potent inoculants in future.
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