Journal of Environmental Biology

Characterization of plant growth promoting rhizobia from root nodule of Mimosa pudica grown in Assam, India

B. Singha¹, P. B. Mazumder¹* and P. Pandey²

1Department of Biotechnology, Assam University, Silchar-788 011, India

2Department of Microbiology, Assam University, Silchar-788 011, India

*Corresponding Author E-mail: pbmazumder65@gmail.com  

Key words

16S rDNA,

Burkholderia,

Mimosa pudica,

PCR-RFLP,

Rhizobia,

Publication Data

Paper received : 08.01.2016

Revised received : 23.05.2016

Re-revised received : 30.09.2016

Accepted : 06.10.2016

Abstract

Aim: Rhizobia are diverse group of soil microorganisms that can form nodule in the roots of leguminous plants and fixes atmospheric nitrogen. In the present study, an attempt was made to characterize the plant growth promoting rhizobia from root nodules of Mimosa pudica grown in Assam.

Methodology: The bacterial isolates were investigated for their phenotypic features. Plant growth promoting abilities of the isolates were determined by conducting different tests. Genotypic characterization of the isolates was carried out by 16S rDNA PCR-RFLP and 16S rDNA gene sequencing. Symbiotic efficiency of the isolates was determined by nodulation test.

Results: The isolates showed similarity in colony morphology, shape and Gram staining, but showed variation in biochemical features. Most of the isolates produced indole acetic acid, ammonia and solubilized inorganic phosphate. Dendrogram based on PCR-RFLP analysis of 16S rDNA gene revealed significant diversity among the isolates. Phylogenetic analysis of the isolates, based on 16S rDNA gene sequences, revealed that SMP2, HMP1 and DMP2 isolates were closely related to Burkholderia mimosarum PAS44, while KMP1 was closely related to Mesorhizobium loti MAFF303099.   

Interpretation: The present study revealed that bacterial strains from Burkholderia genera were the major symbionts of Mimosa pudica grown in Assam. The isolates obtained in the present study possesed plant growth promoting abilities and could be used as a potent inoculants in future. 

Copyright ? 2017 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).