of different environmental conditions on growth and
of some Trichoderma species
Zehra*, M. K. Dubey, M. Meena and R. S. Upadhyay
Mycopathology and Microbial Technology, Centre of Advanced Study in Botany,
Banaras Hindu University, Varanasi-221 005, India
Author E-mail: firstname.lastname@example.org
received : 22.04.2016
received : 14.06.2016
received : 05.08.2016
Accepted : 12.09.2016
Aim: To evaluate the
effect of the different environmental conditions (temperature, pH and salt)
on growth and sporulation of some Trichoderma species.
the present study, Trichoderma spp. were isolated from rhizospheric
soil of various cultivated plants and identified as Trichoderma harzianum,
T. viride, T. hamatum and T. asperellum on the basis of
morphological and conidial characteristics. These identified Trichoderma
spp. were tested in vitro for their antagonistic activity against Fusarium
oxysporum and Alternaria alternata, enzyme production and
tolerance to different environmental conditions such as salt, temperature and
results obtained revealed that out of the four tested Trichoderma
species, maximum inhibition of the growth and spore production of both the
pathogens were found by T. harzianum, with the greatest percent
inhibition was observed for F. oxysporum (68.5%) and A. alternata
(52.93%) in dual cultures. The inhibition rate was also significantly
correlated with the culture filtrate of all the isolates. Significant
chitinase and cellulase acttivities of all Trichoderma isolates have
been recorded during plate assay. T. harzianum exhibited a higher
diameter of clear zone than the other Trichoderma spp. on media
containing chitin and cellulose. Thus, they showed a better cellulolytic and
chitinolytic actitivties as compared to other isolates. However, all these
isolates responded distinctly to different environmental parameters. The most
favourable temperature for growth and sporulation of T. harzianum and T.
viride was found in between 25-40°C, where as for T. asperellum
and T. hamatum, it was 25-35°C. Similarly, the most favourable range
of pH was in between 4.6-7.6 for all the four spp. of Trichoderma.
None of the applied NaCl concentrations except 1000 µM significantly
inhibited mycelial growth of all tested Trichoderma species.
the basis of this study, it can be concluded that T. harzianum is a
potential candidate as a biocontrol agent against various fungal pathogens in
agriculture system under different environmental stresses.
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