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Abstract - Issue Mar 2017, 38 (2) Back
nstantaneous and historical temperature effects on a-pinene
of chickpea proteinase inhibitor on survival and parasitism of root-knot
nematode, Meloidogyne incognita
Gawade*1, 2, A. Sirohi2, A. K. Ganguly2, R.
Kansal3, D. Choudhary2 and R. Koulagi2
1Division of Plant
Quarantine, ICAR-National Bureau of Plant Genetic Resources, New
Nematology, ICAR-Indian Agricultural Research Institute, New Delhi-110012,
Research Centre for Plant Biotechnology, New Delhi-110012, India
Author E-mail: email@example.com
received : 07.12.2016
received : 18.01.2017
received : 23.01.2017
nematodes, Meloidogyne incognita are economically important plant
parasitic nematodes of cultivated crops including vegetables. Eco-friendly
and effective alternate methods are required for management of M.
incognita to reduce use of chemical nematicides. Therefore, present study
was conducted to assess the effect of Cicer arietinum proteinase
inhibitor (CaPI) on survival and parasitism potential of M.
incognita which can further be useful in developing management strategies
against M. incognita.
CaPI gene was cloned in expression vector pET28a and pure CaPI
protein was isolated and characterized. Purified protein was fed (in vitro)
to second stage juveniles (J2s) of M. incognita to the study effect of PI on
survival of J2s. The effect of in vitro feeding of CaPI on parasitism
of M. incognita J2s was also studied by inoculation of protein fed J2s
on tomato plants cv. Pusa Ruby. Tomato plants were observed after 35 days of
inoculation to see the root galls and egg mass formation.
molecular weight of isolated and purified protein was ~30 kDa. In vitro
studies revealed up to 27.73% mortality in M. incognita J2s. The
parasitism potential of CaPI fed J2s was reduced thereby, root gall
and egg mass production on tomato roots were reduced by 77.21 and 86.88%
respectively, after 35 day of inoculation.
In vitro feeding of CaPI
to the M. incognita J2s affected their survival and parasitism
potential. Overall, CaPI has potential in management of M.
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