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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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        Abstract - Issue Mar 2017, 38 (2)                                                                                                             Back

nstantaneous and historical temperature effects on a-pinene

Effect of chickpea proteinase inhibitor on survival and parasitism of root-knot nematode, Meloidogyne incognita


B.H. Gawade*1, 2, A. Sirohi2, A. K. Ganguly2, R. Kansal3, D. Choudhary2 and R. Koulagi2

1Division of Plant Quarantine, ICAR-National Bureau of Plant Genetic Resources, New Delhi-110012, India

2Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi-110012, India

3ICAR-National Research Centre for Plant Biotechnology, New Delhi-110012, India

*Corresponding Author E-mail:




Key words

CaPI gene,


Meloidogyne incognita,

Proteinase inhibitor,





Publication Data

Paper received :           07.12.2016

Revised received :       18.01.2017

Re-revised received :   23.01.2017

Accepted :                  30.01.2017




Aim: Root-knot nematodes, Meloidogyne incognita are economically important plant parasitic nematodes of cultivated crops including vegetables. Eco-friendly and effective alternate methods are required for management of M. incognita to reduce use of chemical nematicides. Therefore, present study was conducted to assess the effect of Cicer arietinum proteinase inhibitor (CaPI) on survival and parasitism potential of M. incognita which can further be useful in developing management strategies against M. incognita.


Methodology: The CaPI gene was cloned in expression vector pET28a and pure CaPI protein was isolated and characterized. Purified protein was fed (in vitro) to second stage juveniles (J2s) of M. incognita to the study effect of PI on survival of J2s. The effect of in vitro feeding of CaPI on parasitism of M. incognita J2s was also studied by inoculation of protein fed J2s on tomato plants cv. Pusa Ruby. Tomato plants were observed after 35 days of inoculation to see the root galls and egg mass formation.


Results: The molecular weight of isolated and purified protein was ~30 kDa. In vitro studies revealed up to 27.73% mortality in M. incognita J2s. The parasitism potential of CaPI fed J2s was reduced thereby, root gall and egg mass production on tomato roots were reduced by 77.21 and 86.88% respectively, after 35 day of inoculation.      


Interpretation: In vitro feeding of CaPI to the M. incognita J2s affected their survival and parasitism potential. Overall, CaPI has potential in management of M. incognita.



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