of plasmid from a Gram negative bacteria
dhakensis strain F2S2-1 and its partial characterization
Nadiga1, 2 V.V. Vaidyanathan1 and T. Thayumanavan2*
International Ltd Bangalore, India
Biotechnology, Dr. G. R Damodaran College of Science, Civil Aerodrome Post,
Coimbatore – 641014, India
Author E-mail: email@example.com
DNA nicking enzyme
received : 30.06.2016
received : 12.08.2016
received : 20.01.2017
Accepted : 28.01.2017
Aim: A plasmid was
isolated from Aeromonas dhakensis strain F2S2-1 to understand their
function in the host. The main objective of the study was to isolate and
sequence the plasmid, predict genes and proteins encoded and to
experimentally prove their biochemical function.
Aeromonas dhakensis strain F2S2-1 plasmid was sequenced by Next
Generation Sequencing (NGS) on Illumina Miseq platform. Bioinformatics
analysis was carried out to predict the putative gene and encoded protein
functions. The NspV like endonuclease was recombinantly expressed in Escherichia
coli, partially purified and characterized for activity on plasmid
sequencing and bioinformatics analysis predicted the presence of NspV like
endonuclease. Recombinant expression, purification and characterization of
NspV like endonuclease revealed the Nicking endonuclease activity of the
protein with plasmid substrates.
NspV like endonuclease described in this study has DNA nicking activity. This
study would help in understanding better survival of Aeromonas sp. in
its ecosystem and the role of host plamids.
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