of dehydrin markers in Capsicum annuum for development of drought
tolerant crops in changing environment
R. Thomas1, M. K. C. Prakash1, M. K. Reddy2,
R. Mahmood3 and P. Mondal1
1Division of Social
Sciences, Indian Institute of Horticultural Research, Bengaluru-560 089,
2Division of Plant
Pathology, Indian Institute of Horticultural Research, Bengaluru-560 089,
Biotechnology and Bioinformatics, Kuvempu University, Shivamogga-577451,
Author E-mail: firstname.lastname@example.org
received : 22.09.2016
received : 12.11.2016
received : 22.12.2016
Accepted : 24.12.2016
Aim: The contribution
of dehydrin is well known in drought stress tolerance in plants. The present
study focus on identifying the markers linked to dehydrin protein for
enhancing drought tolerance in chilli, Capsicum annuum.
publically available 1,15,000 plus EST collection of C. annuum were
explored for identifying the presence of markers. A computer program was
developed to read and analyse large fragments of nucleotide sequences and for
locating the microsatellite repeat motifs in the EST collections of C.
annuum. The program searched for exact motifs, a string of repeated
units, with all possible combinations of motif occurrences.
on conserved protein domains, several ESTs were identified with potential
markers. Among them, one-third were predominated by dehydrin marker also
having high sequence similarity. Three dehydrin markers were found to be
highly conserved and robust, and were associated at high frequency with the
DHN protein. ?
potential dehydrin markers are linked directly to genes that encode trait
specific characteristics and are highly useful for molecular breeding program
for the selection of parental lines and for the development of crops tolerant
to drought stress in changing climate scenario.
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