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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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        Abstract - Issue Mar 2017, 38 (2)                                                                                                             Back



nstantaneous and historical temperature effects on a-pinene

Identification of dehydrin markers in Capsicum annuum for development of drought tolerant crops in changing environment

 

R. R. Thomas1, M. K. C. Prakash1, M. K. Reddy2, R. Mahmood3 and P. Mondal1

1Division of Social Sciences, Indian Institute of Horticultural Research, Bengaluru-560 089, India

2Division of Plant Pathology, Indian Institute of Horticultural Research, Bengaluru-560 089, India

3Department of Biotechnology and Bioinformatics, Kuvempu University, Shivamogga-577451, India

*Corresponding Author E-mail: reenart@hotmail.com

 

 

 

Key words

Capsicum annuum,

Dehydrin marker,

Drought tolerance,

EST sequences,

Plant breeding

 

 

 

Publication Data

Paper received : 22.09.2016

Revised received : 12.11.2016

Re-revised received : 22.12.2016

Accepted : 24.12.2016

 

 

Abstract

Aim: The contribution of dehydrin is well known in drought stress tolerance in plants. The present study focus on identifying the markers linked to dehydrin protein for enhancing drought tolerance in chilli, Capsicum annuum.

 

Methodology: The publically available 1,15,000 plus EST collection of C. annuum were explored for identifying the presence of markers. A computer program was developed to read and analyse large fragments of nucleotide sequences and for locating the microsatellite repeat motifs in the EST collections of C. annuum. The program searched for exact motifs, a string of repeated units, with all possible combinations of motif occurrences.

 

Results: Based on conserved protein domains, several ESTs were identified with potential markers. Among them, one-third were predominated by dehydrin marker also having high sequence similarity. Three dehydrin markers were found to be highly conserved and robust, and were associated at high frequency with the DHN protein. ?    

 

Interpretation: These potential dehydrin markers are linked directly to genes that encode trait specific characteristics and are highly useful for molecular breeding program for the selection of parental lines and for the development of crops tolerant to drought stress in changing climate scenario.

 

 

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