bubble respirometer to monitor microbial activity in
Liu*, K.H. Li and B.Y. Chen
Environmental Engineering and Management, Chaoyang University of Technology,
Taichung, 41349, Taiwan
Author E-mail: firstname.lastname@example.org
Aim: The study aimed to
investigate the relationship between the diesel degradation efficiency in
soil and microbial activity during the wind-driven bioventing remediation.
The study also aimed to compare the indicators of cumulative oxygen uptake
and 24-hr oxygen uptake rate with the analysis results of diesel
self-made wind-driven bioventing equipment was used as passive bioventing
device.In the wind-driven bioventing pilot study for highly diesel
contaminated soil (approximately 20,000 mg kg-1), the bubble
respirometer collocated with the bioslurry method was used to monitor the
microbial activity in soil. The diesel concentration was analyzed by GC/FID.
result of respiration assays showed that the microbial activities of Bacillus
subtilis and Pseudomonas putida, which are capable of degrading
diesel, could be consecutively bioaugmented for two weeks, and that of Achromobacter
xylosoxidans could be consecutively bioaugmented for three weeks after
adding individual microorganism. While appropriate amount of nutrients were
added in the pilot, the cumulative oxygen uptake in the Pseudomonas putida
set elevated from 219 mg-O2 to 328 mg-O2 in one week
that inferred the microbial activity of Pseudomonas putida and which
could be elevated rapidly in a highly diesel contaminated environment.
However, the 24-hour oxygen uptake rate in the Achromobacter xylosoxidans
set could be maintained in the first three weeks between 4.8 mg-O2
hr-1 and 5.0 mg-O2 hr-1 that illustrated the
microbial activity of Achromobacter xylosoxidans, which was the
highest among three microorganisms in the highly diesel contaminated
research results demonstrated a positive correlation between the diesel
degradation efficiency and 24-hour average oxygen uptake rate of
microorganisms during the experiment, suggesting that the 24-hour average
oxygen uptake rate could be used as an indicator of microbial activity.
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