of transgenic peanut plants encoding coat protein
nucleocapsid protein genes for resistance to
streak virus and peanut bud necrosis virus
Patil1,2, R. Thankappan1*, R. Mehta1, R.
Yadav1, A. Kumar1, G.P. Mishra1, J.R.
Dobaria1, P.P. Thirumalaisamy1 and R. K. Jain3
Unit, ICAR-Directorate of Groundnut Research, Junagadh-362 001, India
Genetics and Plant Breeding, College of Agriculture, Junagadh Agricultural
University, Junagadh-362 001, India
3Division of Plant
Pathology, Advanced Centre for Plant Virology, ICAR-Indian Agricultural
Research Institute, New Delhi-110 012, India
Author E-mail: email@example.com
Transgenic plants virus
received : 21.10.2015
received : 12.04.2016
received : 16.07.2016
Accepted : 12.09.2016
engineering of peanuts (Arachis hypogaea L.) via genes encoding for
coat protein (CP gene) of Tobacco streak virus (TSV) and nucleocapsid
protein (NC gene) of a Peanut bud necrosis virus (PBNV) were used to
impart concurrent resistance against the stem and bud necrosis diseases. The
main objective of this study was to determine whether the CP/NC-mediated
resistance strategy could be applied for developing the transgenic peanut
plants, by utilizing CP gene of TSV and NC gene of PNBV.
transgenic lines of peanut cv. K-6 were characterised for integration,
inheritance and expression of transgenes through PCR, RT-PCR and quantitative
PCR (qPCR) analysis. The transgenic plants were artificially challenged with
viruses under confined glasshouse conditions and the load of virus particles
were confirmed using DAC-ELISA, RT-PCR and histopathology in both transgenic and
wild-type (WT) plants.
marker-free transgenic groundnut plants carrying TSV-CP+PBNV-NC genes
witnessed delayed and less intense symptoms after viral inoculation,
suggesting underlying resistance via a coat protein/nucleocapsid-mediated
mechanism and indicated partial/non-durable resistance to TSV and PBNV.
marker free Agrobacterium-mediated transformation technique can be
successfully used to generate transgenic peanuts having resistance to both Ilarvirus
and Tospoviruses.This strategy may be applied to commercially
important crops that are affected by Ilarvirus and Tospoviruses.
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