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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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        Abstract - Issue Mar 2017, 38 (2)                                                                                                             Back



nstantaneous and historical temperature effects on a-pinene

Evaluation of transgenic peanut plants encoding coat protein

and nucleocapsid protein genes for resistance to

tobacco streak virus and peanut bud necrosis virus

 

A.S. Patil1,2, R. Thankappan1*, R. Mehta1, R. Yadav1, A. Kumar1, G.P. Mishra1, J.R. Dobaria1, P.P. Thirumalaisamy1 and R. K. Jain3

1Crop Improvement Unit, ICAR-Directorate of Groundnut Research, Junagadh-362 001, India

2Department of Genetics and Plant Breeding, College of Agriculture, Junagadh Agricultural University, Junagadh-362 001, India

3Division of Plant Pathology, Advanced Centre for Plant Virology, ICAR-Indian Agricultural Research Institute, New Delhi-110 012, India

*Corresponding Author E-mail: radhakrishnan.nrcg@gmail.com

 

 

Key words

Allotetraploid,

Biotic-stress resistance,

necrosis virus,

Tobacco streak,

Transgenic plants virus

 

 

 

Publication Data

Paper received : 21.10.2015

Revised received : 12.04.2016

Re-revised received : 16.07.2016

Accepted : 12.09.2016

 

Abstract

Aim: Genetic engineering of peanuts (Arachis hypogaea L.) via genes encoding for coat protein (CP gene) of Tobacco streak virus (TSV) and nucleocapsid protein (NC gene) of a Peanut bud necrosis virus (PBNV) were used to impart concurrent resistance against the stem and bud necrosis diseases. The main objective of this study was to determine whether the CP/NC-mediated resistance strategy could be applied for developing the transgenic peanut plants, by utilizing CP gene of? TSV and NC gene of PNBV.

?

Methodology: The transgenic lines of peanut cv. K-6 were characterised for integration, inheritance and expression of transgenes through PCR, RT-PCR and quantitative PCR (qPCR) analysis. The transgenic plants were artificially challenged with viruses under confined glasshouse conditions and the load of virus particles were confirmed using DAC-ELISA, RT-PCR and histopathology in both transgenic and wild-type (WT) plants.

 

Results: The marker-free transgenic groundnut plants carrying TSV-CP+PBNV-NC genes witnessed delayed and less intense symptoms after viral inoculation, suggesting underlying resistance via a coat protein/nucleocapsid-mediated mechanism and indicated partial/non-durable resistance to TSV and PBNV.

 

Interpretation: The marker free Agrobacterium-mediated transformation technique can be successfully used to generate transgenic peanuts having resistance to both Ilarvirus and Tospoviruses.This strategy may be applied to commercially important crops that are affected by Ilarvirus and Tospoviruses. 

 

 

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