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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP
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    Abstract - Issue Jan 2017, 38 (1)                                     Back


nstantaneous and historical temperature effects on a-pinene

Screening of phytochemical, antimicrobial and antioxidant activity

of Glycyrrhiza glabra root extract

 

J. Helan Chandra* and Harini Gunasekaran,

Centre for Research, Department of Biotechnology, Jeppiaar Engineering College, Chennai-600 119, India

*Corresponding Author E-mail: helananand@gmail.com

 

 

 

Key words

Antimicrobial activity,

DPPH assay, GC-MS,

Glycyrrhizic acid,

Terpenoids

 

  

Publication Data

Paper received : 26.08.2016

Revised received : 15.11.2016

Re-revised received : 01.12.2016

Accepted : 05.12.2016

 

Abstract

Aim : Glycyrrhiza glabra Linn, an Indian folklore plant, has many therapeutic values. The present study was designed screening of phytochemical components, antioxidant and antimicrobial activity and also GC-MS analysis of the root extract of Glycyrrhiza glabra.

Methodology : Chloroform extract was prepared with powdered root sample of Glycyrrhiza glabra Linn. The crude extracts were characterized by phytochemical screening, DPPH assay and GC-MS analysis followed by antimicrobial assay.

 

Results : Preliminary phytochemical analysis revealed the presence of various bioactive components such as alkaloids, flavonoids, saponins, carbohydrates, tannins, phlobatannins, anthraquinones, terpenoids, cardiac glycosides, glycosides, anthocyanin and sterols. Determined antioxidant activity showed maximum activity of 92.1% at 500 ?gml-1 and the IC50 value was 120.16 ?gml-1. GC-MS study confirmed the presence of seven metabolites such as Pyridine-3-carboxamide, oxime, N-(2-trifluoromethylphenyl)-; Benzonitrile,2-fluoro-4-(4'-propyl[1,1'-bicyclohexyl]-4-yl); Benzamide, 2-bromo-N-[2-(3-fluorophenyl)-5-benzoxazolyl]-; 4-Piperidylbenzilate; 3-Dibenzofuranamine; Lauric anhydride and Carbamicacid,N-(3-chloro-4-methoxyphenyl),glycidyl ester. The crude extract exhibited satisfactory antimicrobial (Escherichia coli and Staphylococcus aureus) and antifungal (Candida albicans and Aspergillus niger) activity.

 

Interpretation : The results of the antimicrobial study revealed efficient activity of crude extract which might be due to the presence of soluble bioactive components and were confirmed by phytochemical analysis, DPPH assay and GC-MS analysis. Thus, it was observed that the roots of Glycyrrhiza glabra could be used for the extraction of medicinally important bioactive compounds.

 

 

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