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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue Nov 2016, 37 (6)                                     Back


nstantaneous and historical temperature effects on a-pinene

Development of gold nanoparticles supported membrane as an

efficient material for protein detection

 

P. Bharathi1,2, N. Balasubramanian1, S. Anitha2 and V. Vijayabharathi2

 1Department of Chemical Engineering, A.C.Tech Campus,Anna University, Chennai-600 025, India

2Department of Biotechnology, KarpagaVinayaga College of Engineering and Technology, Chinnakolambakkam-603 308, India

*Corresponding Author E-mail: nbsbala@annauniv.edu

 

 

 Publication Data

Paper received:

31 October 2015

 

Revised received:

11 July 2016

 

Accepted:

20 August 2016

 

Abstract

A development method for gold nanoparticles (GNPs), supported on nitrocellulose membrane by a simple contact between a GNPs solution was prepared with Tetra aurochloro acid (HAUCL4) solutions precursor. Nitrocellulose membranes are of particular interest in many bimolecular analyses, biosensors, cosmetic industries, especially for detection of specific proteins or peptides and nucleic acid sequences. These kinds of membrane currently suffer from insufficient detection sensitivity and low retention value for proteins less than 5 KDa. These deficiencies can be overcomed by constructing hierarchically structured separation layer on a porous membrane using GNPs colloidal solution. The surface modified membranes were able to effectively detect proteins less than 5 KDa than conventional membranes, since metals have high affinity towards protein. Activities of GNPs supported membrane were studied through western blot experiment and their performances were compared with the conventional membrane. The coated membrane performance increased which was proved by western blot experiment.   

 

 

 Key words

Biosensor, Goldnanoparticles, Nitrocellulosemembrane, Small protein, Western blot 

 

 

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