approach for the optimization? of? keratinase enzyme production from Bacillus
Rajesh, R. Karthick Hari Mathan, S. Rajasekar and B. Anandaraj*
of Biotechnology, Bharathidasan Institute of Technology, Anna University,
Author E-mail: firstname.lastname@example.org
13 October 2015
19 May 2016
20 August 2016
made up of keratin protein are resistant to physical, chemical and
environmental factors. Keratins are classified as heterogeneous proteins due
to the structure and composition of amino acid. Keratinase enzyme can easily
degrade keratin waste and converts into feed stuffs and fertilizers.
Keratinase enzyme is secreted by various microorganisms which mostly include
fungi, actinomycetes and Bacillus. In the present study, Bacillus
cereus KM209198, which was isolated from feather dumped soil for
production of keratinase enzyme was selected. Statistical tools Minitab pro
220.127.116.11 and Design expert 7 were used for optimization of keratinase
production. Plackett-Burman method was used for identifying variables
influencing the enzyme production and further optimized by RSM.
Placket-Burman design, four out of seven variables such as glucose, casein,
magnesium sulphate and pH were identified. These significant variables were
further optimized through hybrid system of response surface methodology.
Optimized concentration was identified as glucose 2.5 g l-1,
casein 7.5 g l-1, MgSO4-2 g-1 and pH 10. Initially
enzyme activity without optimization was 2.2 U ml-1 and after
optimization, the enzyme activity was increased upto 3.5 U ml-1.
cereus, Feather degradation, Keratinase production, Keratinolytic
activity and Microbial degradation
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