Journal of Environmental Biology

Extrication process of chlorogenic acid in Crofton weed and antibacterial mechanism of chlorogenic acid on Escherichia coli

Y. Zheng¹*, J. Liu², M.L. Cao¹, J.M. Deng¹ and J. Kou²

 ¹School of Biological and Chemical Engineering, Panzhihua University, Panzhihua-617 000, China

²Panzhihua Xiyu Biotechnology Co. Ltd, Panzhihua-617 012, China

*Corresponding Author E-mail: zheng_yi_2016@163.com

 Publication Data

Paper received:

21 February 2016

Revised received:

27 April 2016

Re-revised received:

5 May 2016

Accepted:

23 June 2016

Abstract

Crofton weed is a perennial herb and a biological intruding species. The present study firstly used the orthogonal test to compare the differences in extraction of chlorogenic acid in leaves and stems of Crofton weed by using three kinds of solvents, namely water, ethanol and ethyl acetate. The best effect was found by using ethonolic extraction of chlorogenic acid in Crofton weed. Further, by choosing Escherichia coli as test object, in-vitro antibacterial test was conducted to study the antimicrobial activities of chlorogenic acid by testing a series of indexes before and after the interaction between chlorogenic acid and Escherichia coli, to clarify the antibacterial mechanism of chlorogenic acid on Escherichia coli. Finally, by comparing the antibacterial activities of isochlorogenic acid A on Escherichia coli, it was concluded that both chlorogenic acid and isochlorogenic acid A showed antibacterial activities against Escherichia coli, wherein chlorogenic acid had a better antibacterial effect on Escherichia coli than isochlorogenic acid A.   

 Key words

Antibacterial activity, Chlorogenic acid, Crofton weed, Extraction, Orthogonal test

Copyright ? 2016 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).