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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP
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    Abstract - Issue Jul 2016, 37 (4)                                     Back


nstantaneous and historical temperature effects on a-pinene

Studies on vanA gene loci among Methicillin Sensitive

Staphylococcus aureus in rural and urban tertiary care centers

 

 

 

Srinivas Budati1*, S. Narasimha Murty2, Ramprasad Kuncham3, R. Vijayaraghavan1 and Bandaru Narasinga Rao1,4

1Department of Research, Saveetha University, Thandalam, Chennai-602 105, India

2Department of General Medicine, Rajiv Gandhi Institute of Medical Sciences (RIMS), Srikakulam-532 001, India

3Eurofins Genomics India Pvt. Ltd., Whitefield, Bengaluru-560048, India

4Gayatri Vidya Parishad Institute of Health Care and Medical Technology, Madhurawada, Visakhapatnam-530 048, India

*Corresponding Author E-mail: vasmedmicro.sv@gmail.com

 

 

 

Publication Data

Paper received:

16 March 2016

 

Revised received:

14 April 2016

 

Accepted:

30 April 2016

 

Abstract

The present study was carried out identify Vancomycin A (vanA) gene loci among Methicillin Sensitive Staphylococcus aureus (MSSA) isolates collected from rural and urban tertiary care centers. Identification of species was confirmed by Gram staining, Biochemical and PCR methods. In the study, 16665 samples were analyzed out of which 6538 were collected from rural and 10127 from urban regions. In rural, Methicillin Sensitive Staphylococcus aureus (MSSA) constituted 401 (6.1%) and Methicillin Resistant Staphylococcus aureus (MRSA) 531 (8.1%) samples, where as in urban MSSA constituted 524 (5.2%) and MRSA 771 (7.6%) samples. Vancomycin Sensitive Staphylococcus aureus (VSSA) was isolated by agar dilution method. In rural center was 359 (89.5%), Vancomycin Intermediate Resistant Staphylococcus aureus (VISA) was found in 35 (8.7%) and Vancomycin Resistant Staphylococcus aureus (VRSA) in 7 (1.8%) samples, but in case of urban center VSSA was isolated in 451 (86.1%), VISA in 52 (9.9%) and VRSA in 21 (4.0%) samples. VISA and VRSA strains obtained from agar dilution method were further confirmed by E-Test, Disc diffusion and PCR methods. In E-Test VSSA was present in 2 (4.7%) and 1 (1.4%); VISA in 33 (78.6%) and 51 (69.8%), VRSA in 7 (16.7%) and 21 (28.8%) samples, whereas disc diffusion results revealed VISA in 31 (73.8%) and 66 (90.4%) samples, but PCR revealed VISA in32 (76.2%) and 46 (63.0%), VRSA in 7 (16.7%) and 21 (28.8%) samples collected from rural and urban centers. Two strains of vanA locus subjected to PCR amplification, Sanger sequencing and sequencing data were analyzed using BLAST NR database of NCBI genbank and results were compatible with Vancomycin-Resistant Enterococci (VRE) and Vancomycin-Resistant Staphylococci aureus (VRSA). Finally, vanA gene loci could be present in any Gram positive bacteria, which was neither to Methicillin sensitive nor resistant.         

 

 

 Key words

Methicillin sensitive Staphylococci aureus, Vancomycin resistance, VanA gene positivity

 

 

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