Journal of Environmental Biology

Antimicrobial activity of a protein obtained from fruiting body of Lentinula edodes against Escherichia coli and Staphylococcus aureus

Lilia S?nchez-Minutti^1,3, Maura T?llez-T?llez⁴, Saul Tlecuitl-Beristain³, Gerardo Santos-L?pez⁵, Ruben D?az²,

Vijai Kumar Gupta⁶, Gerardo D?az-God?nez²*

¹Postgraduate of Biological Sciences, Autonomous University of Tlaxcala, Tlaxcala, 90000, M?xico

²Laboratory of Biotechnology, Research Center for Biological Sciences, Autonomous University of Tlaxcala, Tlaxcala, 90000, Mexico

³Biotechnology Laboratory, Polytechnical University of Tlaxcala, Tepeyanco, Tlaxcala, 90180, M?xico

⁴Center for Biological Research, Autonomous University of State of Morelos, Morelos, 62209, M?xico

⁵East Center for Biomedical Research, Mexican Social Security Institute, Puebla, 74360, M?xico

⁶Molecular Glycobiotechnology Group, Discipline of Biochemistry, National University of Ireland Galway, Galway, 12074, Ireland

*Corresponding Author E-mail: diazgdo@hotmail.com

Publication Data

Paper received:

16 March 2016

Revised received:

14 April 2016

Accepted:

30 April 2016

Abstract

In thepresent study, a protein extracted from the fruiting body of Lentinula edodes showed growth inhibition of Escherichia coli and Staphylococcus aureus. An aqueous extract from the dry fruiting body for purification process was used. This process was carried out in three stages, isoelectric focusing, ion exchange, preparative electrophoresis. The antimicrobial activity was evaluated by diffusion disc technique. The aqueous extract reported a protein concentration of 57.4 mg l^-1 and showed bacterial inhibition zone of 0.90 and 1.10 cm for E. coli and S. aureus, respectively; the isolated protein (9.69 mg l^-1) formed bacterial inhibition zone of 2.48 cm for E. coli and 2.68 cm for S. aureus. This protein with a purification factor of 16 showed molecular weight of 87.2 kDa and specific activity of 28 cm mg-1. The isolated protein showed 2.8 and 2.4 times higher antimicrobial inhibition zone than aqueous extract for E. coli and S. aureus respectively, and their size was almost equal to that observed with 50 mg l^-1 gentamicin.         

 Key words

Antimicrobial activity, Escherichia coli, Lentinula edodes, Staphylococcus aureus

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