Journal of Environmental Biology

In vitro evaluation of Pseudomonas bacterial isolates from rice phylloplane for biocontrol of Rhizoctonia solani and plant growth promoting traits 

Shamima Akter¹, Jugah Kadir¹*, Abdul Shukor Juraimi² and Halimi Mohd Saud³

¹Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang 43400, Selangor DarulEhsan, Malaysia

²Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang 43400, Selangor DarulEhsan, Malaysia

³Department of Agricultural Technology, Faculty of Agriculture, Universiti Putra Malaysia, Serdang 43400, Selangor DarulEhsan, Malaysia

*Corresponding Author E-mail: kaju@upm.edu.my

Publication Data

Paper received:

10 February 2015

Revised received:

05 August 2015

Accepted:

06 October 2015

Abstract

The ability for biocontrol and plant growth promotion of three Pseudomonas bacterial isolates namely Pseudomonas fluorescens (UMB20), Pseudomonas aeruginosa (KMB25) and Pseudomonas asplenii (BMB42) obtained from rice plants was investigated. Fungal growth inhibition by the isolates ranged from 86.85 to 93.15% in volatile and 100% in diffusible metabolites test. Among the isolates, BMB42 showed fungal growth inhibition significantly in the volatile metabolite test. Isolates UMB20 and BMB42 were able to synthesis chitinase with chitinolytic indices of 13.66 and 13.50, respectively. In case of -1,3-glucanase, all the isolates showed activity to produce this enzyme at varied levels and isolate KMB25 showed significantly highest activity (53.53 ppm). Among the three isolates, KMB25 showed positive response to protease production and all of them were negative to pectinase and lipase and positive to the production of siderophore, and HCN, and were able to solubilize tricalcium phosphate. All the three bacterial isolates were capable of forming biofilm at different levels. Above results suggest that phylloplane Pseudomonas bacterial isolates have potential for antifungal activities and plant growth promotion.         

 Key words

Biocontrol, Growth promotion, Hydrolytic enzymes, Pseudomonas isolates 

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