Journal of Environmental Biology

DNA fingerprinting and genetic diversity studies in wheat genotypes using SSR markers 

Sundeep Kumar*, Vinay Kumar, Pummy Kumari, Kirti, Amit Kumar Singh and Rakesh Singh

Division of Genomic Resources, NBPGR, New Delhi-110012, India

*Corresponding Author E-mail: sundeep@nbpgr.ernet.in, sundeep.kumar@icar.gov.in

Publication Data

Paper received:

30 October 2015

Revised received:

04 January 2016

Accepted:

09 January 2016

Abstract

Fifty four wheat genotypes comprising of 41 Indian origin and 13 exotic genotypes were characterized using 39 polymorphic SSR markers for DNA fingerprinting and extent of genetic diversity. A total of 112 alleles ranging from 1 (Barc1, Barc26 and Barc147) to 7 alleles (Barc77) were generated with an average of 2.87 per SSR marker. The PIC value of SSR markers ranged from 0.03 to 0.49 with 0.29 mean PIC value indicating lower level of genetic diversity among genotypes. Similarity values ranged from 22.8% (between MP-4161 and K-424) to 78.7 % (between GP-350 and GP-361) with an average of 51.23 %. UPGMA based cluster analysis, broadly grouped 54 genotypes into four clusters were represented as A, B, C and D. Cluster D included maximum number of genotypes (43) which was further divided into seven sub-clusters. Out of thirteen exotic genotypes used in the present study, six were included in sub-cluster D4. However, two exotic genotypes each were sub-clustered with Indian genotypes into three sub-clusters, D-2, D-6 and D-7 revealing their genetic similarity with genotypes of Indian origin. Genotypes from same origin were usually clustered together, e.g. K-65 and K-68 belonged to Kanpur region were clustered into 'Cluster B'. Similarly, MP-4115, MP-4131, MP-4136 and MP-4010 released for Madhya Pradesh were clustered into 'D-3' sub-cluster. Present study also showed the potentiality of SSR markers for study of genetic diversity and DNA fingerprinting in wheat. ????????  

 Key words

Cultivar identification, DNA Fingerprinting, PIC values, SSR markers, Wheat

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