Journal of Environmental Biology

Study on DNA damaging effects of 4-nonylphenol using erythrocytes from peripheral circulation, gill and kidney of fish Channa punctatus 

Madhu Sharma and Pooja Chadha*

Department of Zoology, Guru Nanak Dev University, Amritsar-143 005, India

*Corresponding Author E-mail: poojachadha77@yahoo.co.in

Publication Data

Paper received:

11 October 2014

Revised received:

04 February 2015

Accepted:

01 April 2015

Abstract

The present study aimed to evaluate the genotoxic effect of 4-nonylphenol (NP) on blood cells of fish Channa punctatus. Fish were exposed to three sublethal concentrations (0.15 mg l^-1; 0.31 mg l^-1? and 0.63 mg l^-1) of 4-NP for 24, 48, 72 and 96 hrs. Blood cells from kidney, gills and peripheral circulation were analyzed for the presence of micronuclei and other changes in the erythrocytes. Significant changes were observed in all the experimental groups tested when compared with control. Highest genotoxicity was observed in blood cells obtained? from gills (MN-2.92%, aberrant cell- 70.64%), followed by kidney (MN-1.34%, aberrant cells-64.94%), were least effect was observed in blood cells obtained from peripheral circulation (MN-0.88%, aberrant cells- 46.27%).Therefore, micronucleus test performed on blood cells obtained from different sources showed that gills were more sensitive as compared to peripheral blood and kidney revealing genotoxic effect of 4-NP on fish C. punctatus. ????????  

 Key words

Blood cells, Channa, Genotoxicity, Micronucelus, 4-nonylphenol

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