Journal of Environmental Biology

Production, partial purification and characterization of xylanase using Nicotiana tabacum leaf dust as substrate 

Komal P. Acharya* and Prateek Shilpkar

Department of Microbiology, M.D. Gramseva Mahavidyalaya, Gujarat Vidyapeeth, Sadra-382 320, India

*Corresponding author E-mail : komal.ach13@gmail.com

Publication Data

Paper received:

27 October 2014

Revised received:

21 April 2015

Accepted:

09 June 2015

Abstract

Isolated Bacillus sp. was used in the present study for production of xylanase from Nicotiana tabacum leaf dust. The strain was able to give a maximum of 1.77 Uml^-1 xylanase activity under optimized fermentation conditions which was further increased upto 2.77 Uml^-1 after extraction and partial purification of enzyme. After partial purification, the enzyme was characterized and it gave the highest xylanase activity at pH 7.0, when 0.2 ml enzyme was incubated with 2.0% substrate (Nicotiana tabacum leaf dust) for 60 min at 60⁰C. Saccharification study of Nicotiana tabacum leaf dust with partially purified enzyme revealed that 18.4% reducing sugar was released in 20 hrs incubation, and TLC and HPTLC analysis showed that xylose and glucose sugars were obtained after hydrolysis of substrate. FTIR analysis confirmed decomposition of substrate.? ??????  

 Key words

Characterization, Nicotiana tabacum, Saccharification, Submerged fermentation, Xylanase.

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