comparative study on decolorization of reactive azo and
dyes by free/immobilized pellets of
versicolor and Funalia trogii
Yildirim* and Ozfer Yesilada
Section, Department of Biology, Art and Science Faculty, Inonu University,
Author E-mail: firstname.lastname@example.org
23 May 2014
23 November 2014
29 December 2014
20 March 2015
objective of the present study was to investigate decolorization of Acid Blue
74 and Reactive Blue 198 dyes by free and immobilized white rot fungal
pellets in order to confirm the possibility of practical application via
repeated-batch cultivation. Decolorization studies were conducted using free
pellets (FP), fungal cells immobilized on activated carbon (IFCAC) and
pinewood (IFCP), and also fungal cells entrapped in alginate beads (FCEAB).
No additional nitrogen and carbon source was used and high decolorization
rates were achieved in only dye-contained media without pH adjustment. Acid
Blue 74 was decolorized 96 and 94% within 2 hr by Trametes versicolor
and Funalia trogii free pellets, respectively. These values were 87
and 84% for Reactive Blue 198, in this respect. Immobilization of fungal
cells on pinewood increased the usability of pellets and the average
decolorization efficiency of both dyes. The micro environment changed in the
presence of pinewood and increased the stability of immobilized pellets.
Decolorization was performed rapidly and efficiently. Laccase activity
enhanced with availability of pinewood, and high laccase production with F.
trogii was obtained. After separation by sodium dodecyl
sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), the molecular weight
of T. versicolor and F. trogii laccase bands was determined 64
and 61 kDa approximately. Green bands were obtained by the activity staining
process with laccase substrate (ABTS) after gel renaturation step. ?????
Immobilization, Laccase, Textile dye, White rot fungi
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