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Abstract - Issue Nov 2015, 36 (6) Back
nstantaneous and historical temperature effects on a-pinene
and characterization of luminescent bacterium
Mohd. Izuan Effendi Halmi1, Siti Aqlima Ahmad1*, Mohd.
Yunus Shukor1 and Mohd. Arif Syed1
Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti
Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
Chemistry, International Islamic University Malaysia (IIUM), Jalan Sultan
Haji Ahmad Shah, Indera Mahkota, 25200 Kuantan, Pahang Malaysia
author E-mail : email@example.com
14 November 2013
09 May 2014
08 December 2014
is based on the inhibition of luminescence of the bacterium Vibrio fischeri
by the toxicants. This technique has been accepted by the USEPA (United
States Environmental Protection Agency) as a biomonitoring tool for
remediation of toxicants such as hydrocarbon sludge. In the present study, a
luminescent bacterium was isolated from yellow striped scad (Selaroides
leptolepis) and was tentatively identified as Vibrio sp. isolate MZ. This
aerobic isolate showed high luminescence activity in a broad range of
temperature from 25 to 35 °C. In addition, optimal conditions for high
bioluminescence activity in range of pH 7.5 to 8.5 and 10 gl-1 of
sodium chloride, 10 gl-1 of peptone and 10 gl-1 of sucrose as carbon source.
Bench scale biodegradation 1% sludge (w/v) was set up and degradation was
determined using gas chromatography with flame ionised detector (GC-FID). In
this study, Rhodococcus sp. strain AQ5NOL2 was used to degrade the
sludge. Based on the preliminary results obtained, Vibrio sp. isolate MZ was
able to monitor the biodegradation of sludge. Therefore, Vibrio sp. isolate
MZ has the potential to be used as a biomonitoring agent for biomonitoring of
sludge biodegradation particularly in the tropical ranged environment.
Biodegradation, Isolation, Sludge, Tropics, Vibrio sp.
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