Journal of Environmental Biology

Isolation and characterization of luminescent bacterium

for sludge biodegradation

Maryam Zahaba^1,2, Mohd. Izuan Effendi Halmi¹, Siti Aqlima Ahmad¹*, Mohd. Yunus Shukor¹ and Mohd. Arif Syed¹

¹Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

²Department of Chemistry, International Islamic University Malaysia (IIUM), Jalan Sultan Haji Ahmad Shah, Indera Mahkota, 25200 Kuantan, Pahang Malaysia

*Corresponding author E-mail : aqlima@upm.edu.my

  Publication Data

Paper received:

14 November 2013

Revised received:

09 May 2014

Accepted:

08 December 2014

Abstract

Microtox is based on the inhibition of luminescence of the bacterium Vibrio fischeri by the toxicants. This technique has been accepted by the USEPA (United States Environmental Protection Agency) as a biomonitoring tool for remediation of toxicants such as hydrocarbon sludge. In the present study, a luminescent bacterium was isolated from yellow striped scad (Selaroides leptolepis) and was tentatively identified as Vibrio sp. isolate MZ. This aerobic isolate showed high luminescence activity in a broad range of temperature from 25 to 35 ?C. In addition, optimal conditions for high bioluminescence activity in range of pH 7.5 to 8.5 and 10 gl^-1 of sodium chloride, 10 gl-1 of peptone and 10 gl-1 of sucrose as carbon source. Bench scale biodegradation 1% sludge (w/v) was set up and degradation was determined using gas chromatography with flame ionised detector (GC-FID). In this study, Rhodococcus sp. strain AQ5NOL2 was used to degrade the sludge. Based on the preliminary results obtained, Vibrio sp. isolate MZ was able to monitor the biodegradation of sludge. Therefore, Vibrio sp. isolate MZ has the potential to be used as a biomonitoring agent for biomonitoring of sludge biodegradation particularly in the tropical ranged environment.   

 Key words

Bioassay, Biodegradation, Isolation, Sludge, Tropics, Vibrio sp. 

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