Journal of Environmental Biology

Purification and characterization of an extracellular cellulase from Anoxybacillus gonensis O9 isolated from geothermal area in Turkey

Berna Genc¹, Hayrunnisa Nadaroglu²*, Ahmet Adiguzel³ and Ozkan Baltaci³

¹Department of Genetic and Bioengineering, Faculty of Engineering and Life Sciences, Gumushane University, 29100, Gumushane, Turkey

²Department of Food Technology, Erzurum Vocational Training School, Ataturk University, 25240 Erzurum, Turkey

³Department of Molecular Biology and Genetics, Faculty of Science, Ataturk University, 25240 Erzurum, Turkey

*Corresponding Author E-mail: hnisa25@atauni.edu.tr

Publication Data

Paper received:

19 July 2014

Revised received:

30 December 2014

Accepted:

20 March 2015

Abstract

In the present study, cellulase was purified and characterized from Anoxybacillus gonensis (Gen bank Number: KM596794) which was isolated and characterized from Agri Diyadin Hot spring. It was found to synthesize cellulase which had a wide range of industrial applications. Twenty four-hour-cultured bacteria induced cellulase production and specific activities during the purification steps were 1.47, 81.06 and 109.4 EU mg^-1 protein at crude extract, ammonium sulphate precipitated and DEAE-Sephadex purification steps. The highest enzyme activity was observed at 50oC and the optimum range of pH was 3-10. Molecular weight of enzyme was determined approximately 40kDa. The kinetic parameters of cellulase against carboxymethylcellulose (CMC) were 153.4 ?mol min^-1 mg for V_max and 0.46mM for K_m. Among effectors of the enzyme, Zn²⁺, Ca²⁺, Co²⁺ and EDTA decreased enzyme activity. ????  

 Key words

Anoxybacillus gonensis, 16S rRNA sequencing, Cellulase, Characterization 

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