JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

About Journal
    Editorial Board
    Reviewer Panel
    RnD Division
    Subscription Info
    Contact Journal
Read Journal
    Current Issue
    Journal Archives
For Authors
    Authoring Guidelines
    Publication Process
    Track Paper Status

Search the Journal web-site through Google:

        Abstract - Issue Nov 2015, 36 (6)                                                                                                             Back

nstantaneous and historical temperature effects on a-pinene

Purification and characterization of an extracellular cellulase from Anoxybacillus gonensis O9 isolated from geothermal area in Turkey


Berna Genc1, Hayrunnisa Nadaroglu2*, Ahmet Adiguzel3 and Ozkan Baltaci3

1Department of Genetic and Bioengineering, Faculty of Engineering and Life Sciences, Gumushane University, 29100, Gumushane, Turkey

2Department of Food Technology, Erzurum Vocational Training School, Ataturk University, 25240 Erzurum, Turkey

3Department of Molecular Biology and Genetics, Faculty of Science, Ataturk University, 25240 Erzurum, Turkey

*Corresponding Author E-mail:




Publication Data

Paper received:

19 July 2014


Revised received:

30 December 2014



20 March 2015



In the present study, cellulase was purified and characterized from Anoxybacillus gonensis (Gen bank Number: KM596794) which was isolated and characterized from Agri Diyadin Hot spring. It was found to synthesize cellulase which had a wide range of industrial applications. Twenty four-hour-cultured bacteria induced cellulase production and specific activities during the purification steps were 1.47, 81.06 and 109.4 EU mg-1 protein at crude extract, ammonium sulphate precipitated and DEAE-Sephadex purification steps. The highest enzyme activity was observed at 50oC and the optimum range of pH was 3-10. Molecular weight of enzyme was determined approximately 40kDa. The kinetic parameters of cellulase against carboxymethylcellulose (CMC) were 153.4 µmol min-1 mg for Vmax and 0.46mM for Km. Among effectors of the enzyme, Zn2+, Ca2+, Co2+ and EDTA decreased enzyme activity.       



 Key words

Anoxybacillus gonensis, 16S rRNA sequencing, Cellulase, Characterization 



Copyright © 2015 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).