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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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        Abstract - Issue Nov 2015, 36 (6)                                                                                                             Back



nstantaneous and historical temperature effects on a-pinene

Purification and characterization of an extracellular cellulase from Anoxybacillus gonensis O9 isolated from geothermal area in Turkey

 

Berna Genc1, Hayrunnisa Nadaroglu2*, Ahmet Adiguzel3 and Ozkan Baltaci3

1Department of Genetic and Bioengineering, Faculty of Engineering and Life Sciences, Gumushane University, 29100, Gumushane, Turkey

2Department of Food Technology, Erzurum Vocational Training School, Ataturk University, 25240 Erzurum, Turkey

3Department of Molecular Biology and Genetics, Faculty of Science, Ataturk University, 25240 Erzurum, Turkey

*Corresponding Author E-mail: hnisa25@atauni.edu.tr

 

 

 

Publication Data

Paper received:

19 July 2014

 

Revised received:

30 December 2014

 

Accepted:

20 March 2015

 

Abstract

In the present study, cellulase was purified and characterized from Anoxybacillus gonensis (Gen bank Number: KM596794) which was isolated and characterized from Agri Diyadin Hot spring. It was found to synthesize cellulase which had a wide range of industrial applications. Twenty four-hour-cultured bacteria induced cellulase production and specific activities during the purification steps were 1.47, 81.06 and 109.4 EU mg-1 protein at crude extract, ammonium sulphate precipitated and DEAE-Sephadex purification steps. The highest enzyme activity was observed at 50oC and the optimum range of pH was 3-10. Molecular weight of enzyme was determined approximately 40kDa. The kinetic parameters of cellulase against carboxymethylcellulose (CMC) were 153.4 ?mol min-1 mg for Vmax and 0.46mM for Km. Among effectors of the enzyme, Zn2+, Ca2+, Co2+ and EDTA decreased enzyme activity. ????  

 

 

 Key words

Anoxybacillus gonensis, 16S rRNA sequencing, Cellulase, Characterization 

 

 

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