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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue Sep 2015, 36 (5)                                     Back

nstantaneous and historical temperature effects on a-pinene

Decolourization of azo, heterocyclic and reactive dyes using spent mycelium substrate of Hypsizygus ulmarius


R. Ranjini and T. Padmavathi*

Department of Microbiology, Centre for Post Graduate Studies, Jain University, Bangalore-560 011, India

*Corresponding Author E-mail:





Publication Data

Paper received:

26 March 2014


Revised received:

27 October 2014



14 January 2015



Cultivation of Hypsizygus ulmarius to generate spent mycelium substrate (SMS) for dye decolourization gave better yield, biological efficiency, fruit width and moisture content (145 gm, 33%, 4 cm, 91%) on paddy straw as compared to coconut husk (59 gm, 21%, 3 cm, 90%). Solid-phase decolourization of Congo red (Azo dye) and Methylene blue (Heterocyclic dye) showed that maximum decolourization (3.31), measured as Decolourization Index, occurred at dye concentration of 25 mg l-1, while in Solochrome black (Reactive dye), it was at 100 mg l-1 (1.7). Time taken for maximum decolourization was 10 days in Congo red and Solochrome black; 20 days in Methylene blue. Decolourization Index was maximum in Methylene blue (3.1), followed by Congo red (1.9) and Solochrome black (1.2). Liquid-phase decolourization of Methylene blue and Solochrome black showed that maximum decolourization (62.5%) measured as percent decolourization occurred at 25 mg l-1, while it was at 50 mg l-1 (36%) for Congo red. Time taken for maximum decolourization for all three dyes was 10 days. During this period, the percent decolourization was maximum in Methylene blue (91.3%), followed by Solochrome black (82.2%) and Congo red (79.7%). Decolourization potential in solid-phase was observed till 100 mg l-1 and day 25 for all the three dyes, however, in liquid-phase it was observed till 50 mg l-1 and day 20 for Congo red, 75 mg l-1 and day 10 for Solochrome black, 100 mg l-1 and day 20 for Methylene blue. Maximum laccase was produced on day 25 during decolourization of 25 mg l-1 Congo red, while maximum Manganese peroxidase was noted on day 20 at 50 mg l-1 Congo red.   



 Key words

Azo dye, Heterocyclic dye, Hypsizygus ulmarius, Reactive dye, Spent mycelium substrate 



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