Journal of Environmental Biology

Genetic differentiation of wild and hatchery populations of

Indian major carp Cirrhinus cirrhosus in Bangladesh

Md. Rajib Sharker¹, Muhammad Abu Bakar Siddik¹*, Ashfaqun Nahar² , Md. Shahjahan^3,4 and Abdullah Al Faroque⁵

¹Department of Fisheries Biology and Genetics, Patuakhali Science and Technology University, Patuakhali-8602, Bangladesh

²Department of Marine Fisheries and Oceanography, Patuakhali Science and Technology University, Patuakhali-8602, Bangladesh

³Sado Marine Biological Station, Faculty of Science, Niigata University, Sado, Niigata-952-2135, Japan

⁴Department of Fisheries Management, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

⁵Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

*Corresponding Author E-mail: siddik@pstu.ac.bd

Publication Data

Paper received:

20 October 2014

Revised received:

17 April 2015

Accepted:

05 June 2015

Abstract

Genetic differentiation of Cirhinus cirrhosus, three river (Halda, Jamuna and Padma) and six hatchery (Brahmaputra, Reliance, Kazi, Rupali, Bismillah and Pacific) populations covering a wide range of geographical distribution of this species were analyzed by allozyme electrophoresis. Four enzymes encoded by 7 loci were screened, where 3 loci were polymorphic (Mdh-1*, Mdh-2*, Gpi-1*). The results showed that polymorphic loci per population (28.57%), mean proportion of heterozygous loci per individual (10.000), mean number of alleles per locus (1.286), and relative gene diversity (0.148) in river populations were higher than those for hatchery populations (23.81, 6.677, 1.238, 0.140, respectively). Also, the observed heterozygosity (Ho) and expected heterozygosity (He) in river populations (0.086 and 0.112, respectively) were higher than those of hatchery populations (0.046 and 0.106, respectively). The pairwise population differentiation (F_ST) values showed a lower level of genetic differentiation between hatchery and river population pairs. The unweighted pair-group method with arithmetic mean dendrogram of Nei's genetic distances showed relationship between genetic distance and geographic distance. Population were clustered into three groups: Halda, Padma and Jamuna river population in one group; Brahmaputra, Reliance and Kazi hatchery populations in second group and Rupali, Bismillah and Pacific hatchery population in third group. The present study revealed that there were obvious genetic variations among the natural and hatchery populations. ???   

 Key words

Allozyme electrophoresis, Allele frequency, Genetic diversity, Polymorphic loci

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