Biodegradation
of furfural by Bacillus subtilis strain DS3
Dan Zheng,
Jianguo Bao* and Jueming Lu and Quanxi Lv
State Key
Laboratory of Biogeology and Environmental Geology, China University of
Geosciences, Wuhan 430074, People's Republic of China
*Corresponding
Author?s Email : bjianguo@cug.edu.cn
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Publication Data
Paper received:
21 May 2014
Revised received:
30 September 2014
Re-revised received:
01 January 2015
Accepted:
07 February 2015
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Abstract
An
aerobic bacterial strain DS3, capable of growing on furfural as sole carbon
source, was isolated from actived sludge of wastewater treatment plant in a
diosgenin factory after enrichment. Based on morphological physiological
tests as well as 16SrDNA sequence and Biolog analyses it was identified as
Bacillus subtilis. The study revealed that strain DS3 utilized furfural, as
analyzed by high-performance liquid chromatography (HPLC). Under following
conditions: pH 8.0, temperature 35℃, 150
rpm and 10% inoculum, strain DS3 showed 31.2% furfural degradation. Furthermore,
DS3 strain was found to tolerate furfural concentration as high as 6000 mgl-1.
The ability of Bacillus subtilis strain DS3 to degrade furfural has been
demonstrated for the first time in the present study. ?
Key
words
Bacillus
subtilis, Biodegradation, Furfural
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