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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue Jul 2015, 36 (4)                                     Back

nstantaneous and historical temperature effects on a-pinene

Sulfide-based mixotrophic denitrification for treatment of sulfur, nitrogen and carbon-contaminated wastewater


Li Wei1, Liang Xiao2, Liu Lei1 and Lin Jianguo1*

1College of Environmental Science and Engineering, Dalian Maritime University, Dalian-116026, China

2College of Transportation Equipment and Ocean Engineering, Dalian Maritime University, Dalian-116026, China

*Corresponding Author?s Email :



Publication Data

Paper received:

08 June 2014


Revised received:

30 September 2014


Re-revised received:

01 January 2015



07 February 2015



Wastewater contaminated by sulfur, nitrogen and carbon compounds was treated by sulfide-based denitrification process in an expanded granular sludge bed packed with porous sponge. In influent, nitrate and nitrite served as electron acceptors, while sulfide and organic carbon served as electron donors. Both sulfide-based autotrophic denitrification and heterotrophic denitrification were found in the bioreactor. The percentage of heterotrophic denitrification was 36.5%, and the removal rates of sulfide, nitrate, nitrite and organic carbon were 99%, 99%, 95.5% and 80% respectively, which actualized the simultaneous mixotrophic denitrification and desulfurization. The effect factors such as organic carbon category, organic carbon loading rate and nitrite loading rate were also investigated. The 50mgCl-1d-1 of glucose and 25mgCl-1 d-1 acetate sodium were found good for sulfide-based denitrification, while the suitable loading rate of nitrite was 50mgNl-1d-1.     



 Key words

Denitrification, Desulfurization, Mixotrophic, Nitrite, Organic carbon



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