Journal of Environmental Biology

Response surface methodology for standardisation of lignocellulosic biomass saccharification efficiency of NSF-2 fungus isolate

Namita Singh¹*, Anita Devi¹, Sandeep Kumar¹ and A. Verma²

¹Department of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar- 125 001, India

²Department of instrumental Engineering, Kurukshetra University, Kurukshetra-136 119, India

*Corresponding Author?s E-mail: namitasingh71@gmail.com

  Publication Data

Paper received:

05 May 2013

Revised received:

11 November 2014

Re-revised received:

16 July 2014

Accepted:

28 December 2014

Abstract

Lignocellulosic biomass can be used as a low cost substrate for cellulase production. In the present study an attempt was made to optimize physico- chemical condition standardization for simultaneous cellulase production by NSF-2 fungal isolate, using wheat straw as a substrate and enzymatic hydrolysis of wheat straw by cellulase produced in-vitro by NSF-2 fungal isolate. Experiments showed maximum saccharification after 5^th day of incubation. Optimum pH and temperature for saccharification were 5 and 30˚C respectively. Further optimization was carried out by response surface methodology using Box-Behnken design (BBD). BBD was designed with different combinations of three variables (peptone as nitrogen source, lignocellulosic biomass as substrate and Tween? 80 as surfactant, each at three levels 1 g l^-1, 2 g l^-1, 3 g l^-1, 0.5 g l^-1, 2.5 g l^-1, 5 g l^-1 and (0.05 %, 0.10%, 0.15%) respectively. The model computed for R² value (99.55%) indicated that this was appropriate and could be useful in predicting the effect of the studied variables. Experimental results showed maximum saccharification at the middle concentration of peptone and substrate i.e. 2 g l^-1 and 2.5 g l^-1 respectively. Surfactant did not show much significant result. ??  

 Key words

NSF- 2 fungal isolate, Response surface methodology, Saccharification efficiency

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