Journal of Environmental Biology

Proficiency of biosynthesized silver nanoparticles as a fungicide against selected damping off causing fungi

Ahmed Abdel-Megeed^1,3, Essam N. Sholkamy^1,2*, Alarfaj A. Abdullah¹, Ashraf A. Mostafa¹, Hussein H. Alkhamis¹,

Ahmed S. Abdel-Aty⁴ and M. MR. M. Ahmed⁵

¹Department of Botany and Microbiology, Faculty of Science, King Saud University, 2455, Riyadh 1145, Riyadh, Saudi Arabia

²Department of Botany and Microbiology, Faculty of Science, Beniseuf University, 62511, Beniseuf, Egypt

³Department of Plant Protection, Faculty of Agriculture, Saba Basha, Alexandria University, 21531, Alexandria, Egypt

⁴Department of Pesticide Chemistry and Technology, Faculty of Agriculture, Alexandria University, 21526, Alexandria, Egypt

⁵Botany Department, Faculty of Science, Assiut University, New Valley Branch, 71515, Assiut, Egypt

*Corresponding Author?s E-mail: essam_92003@yahoo.com

 Publication Data

Paper received:

22 September 2014

Revised received:

05 February 2015

Accepted:

20 March 2015

Abstract

The present study aimed at studying the fungicidal properties of nano-sized silver particles (AgNPs), biosynthesized from Nocardiopsis dassonvillei at 10, 20, 40, 80 and 160 ?g ml^-1 against selected damping off causing fungi. Silver nanoparticles (AgNPs) were characterized by energy dispersive spectroscopy (EDS) and transmission electron microscopy (TEM). TEM micrographs revealed extracellular formation of spherical nanoparticles with an average size of 25.8 nm. All the tested fungi were highly inhibited by the biosynthesized AgNPs.? Its effective concentrations inhibiting 50% of the fungal growth (EC₅₀) values on F. solani, A. alternata, F. oxysporum and R. solani were 1.25, 3.23, 4.3 and 9.2 ?g ml^-1, respectively. The present study proved that biosynthesized AgNPs as a promising fungicide against the treated fungi.      

 Key words

Alternaria, Damping off, Fungicide, Fusarium, Rhizoctonia, Silver nanoparticles 

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