Journal of Environmental Biology

Genotoxic effects of cadmium on tolerant and sensitive

wheat cultivars 

Fatma Mutlu¹* and Birol Mutlu²

¹İn?n? University, Faculty of Education, Department of Science Teaching, 44280, Malatya, Turkey

²İn?n? University, Faculty of Science and Arts, Department of Biology, 44280 Malatya, Turkey

*Corresponding Author?s E-mail: fatma.mutlu@inonu.edu.tr

 Publication Data

Paper received:

21 May 2014

Revised received:

29 September 2014

Accepted:

24 December 2014

Abstract

In the present study, genotoxic properties of cadmium (Cd) in seedlings of wheat (Triticum vulgare L.) cultivars, chosen as cadmium tolerant (S?nmez-2001) and sensitive (Quality) seedlings, were evaluated by using randomly amplified polymorphic DNA (RAPD-PCR). After cadmium treatment, significant changes were observed on RAPD profiles of these cultivars. Genomic template stability (GTS) and cluster of similarity (UPGMA) were studied. GTS rates demonstrated that cv. Quality, which is a sensitive cultivar, was more affected. Furthermore, the distinguishing specific markers between tolerant and sensitive cultivars were determined. It can be concluded that DNA polymorphisms detected by RAPD-DNA, can be considered as a powerful biomarker assay for detection of the genotoxic effects of Cd stress. ?    

 Key words

Cadmium, Genomic Template Stability, Genotoxic effects, RAPD, Wheat

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