Delignification
of cotton gin waste and its optimization by using
white
rot fungus Pycnoporus cinnabarinus
Shitarashmi Sahu
and Krishna Pramanik*
Department of
Biotechnology and Medical Engineering, National Institute of Technology,
Rourkela-769 008, India
*Corresponding
Author?s E-mail: kpr@nitrkl.ac.in
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Publication
Data
Paper received:
10 January 2013
Revised received:
13 May 2014
Re-revised received:
25 August 2014
Accepted:
21 October 2014
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Abstract
The
present study investigated the effect of fungal pretreatment on cotton gin
waste by solid and submerged state of cultivation and screening potential
fungus for delignification. Cotton gin waste was treated with white rot fungi
namely, Trametes pubscens, Pleurotus ostreatus, Pycnoporus cinnabarinus
and Phanerochaete chrysosporium to separate cellulose and hemicellulose
components by degrading lignin from a complex mixture of the above three
components using their secreted enzymes. In the delignification process,
solid state cultivation (SSC) was found to be more effective than submerged
cultivation (SMC). Among the four fungi used in the study, Pycnoporus
cinnabarinus showed better result in achieving lignin removal of 55.2 and
40.2% in solid and submerged cultivation respectively. The corresponding
cellulose and hemicellulose reduction was determined as 61.9 and 70% in SSC,
whereas their value in SMC was 44% cellulose and 56.3% hemicellulose. The
confirmation of delignification process with respect to fungal pretreated and
untreated cotton gin waste was assessed using FTIR, XRD and SEM analysis.
Optimization of parameters for Pycnoporus cinnabarinus further showed
substantial improvement in lignin removal i.e., 60% in SSC at pH, shaking
speed and temperature of 4.5, 138 rpm and 320C respectively. ?
Key
words
Cotton
gin waste, White rot fungi, Lignin, Cellulose, Hemicellulose
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