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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue May 2015, 36 (3)                                     Back


nstantaneous and historical temperature effects on a-pinene

Chlorpyrifos induced gene mutations in Culex quinquefasciatus 

 

Preety Bhinder

Department of Zoology, Punjab University, Chandigarh-160 014, India

Author?s Email : preety.bhinder@yahoo.com

 

 

 

 Publication Data

Paper received:

16 April 2013

 

Revised received:

22 September 2013

 

Re-revised received:

16 December 2013

 

Accepted:

05 May 2014

 

Abstract

Mutagenicity of chlorpyrifos was evaluated by using the genetic material of mosquito Culex quinquefasciatus. Second instar larvae were treated with LC20 of chlorpyrifos for 24 hrs and induced mutations in the sequence of mitochondrial 16S rRNA gene (543bp) were studied from restriction patterns generated with AluI, PacI and PsiI restriction endonucleases. Variation in the number and size of digested fragments were observed from treated individual when compared with control, showing that the restriction enzymes created a cut at unrelated locations. In addition, sequences of 16S gene from control and treated individuals were also used to confirm the RFLP patterns. When 16S gene sequences of control and treated individuals were compared, induced mutations in the form of deletion, insertion and substitution of bases were observed in chlorpyrifos treated individual. It was found that after chlorpyrifos treatment, 16S gene sequence suffered 4 deletions, 8 insertions and 23 substitutions.   

 

 

 Key words

Chlorpyrifos, Culex quinquefasciatus, Mutagenicity

 

 

 

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