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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue May 2015, 36 (3)                                     Back


nstantaneous and historical temperature effects on a-pinene

Influence of Endocel and Rogor on serum free amino acid and total protein level in Clarias batrachus (Linn.) 

 

Prakriti Verma1*, Bipin Bihari Mishra2 and Prabha Rani1

1Post Graduate Department of Zoology, Patna University, Patna - 800 005, India

2Post Graduate Department of Biochemistry, Patna University, Patna - 800 005, India

*Corresponding Author?s E-mail: drprakriti@gmail.com

 

 

 

 Publication Data

Paper received:

25 June 2013

 

Revised received:

29 January 2014

 

Re-Revised received:

12 June 2014

 

Accepted:

17 June 2014

 

Abstract

The present work was conducted to evaluate the serum free amino acids and total protein level in control and pesticide exposed fish. The fresh water catfish Clarias batrachus were exposed to single (1.5 ?l l-1 Rogor and 0.008 ?l l-1 Endocel) and combined (1?l l-1 Rogor + 0.004 ?l l-1 Endocel) dose of Rogor and Endocel pesticides for 21 days. Every week blood was collected, centrifuged and serum was separated to estimate free amino acids by thin layer chromatography. Amino acids were located on chromatogram with 0.1% ninhydrin solution in acetone. Spectrophotometric reading found that each chromatogram revealed 4-5 fractions with a wide range of amino acid such as aspartic acid, glutamic acid, phenylalanine, serine, tyrosine, alanine, methionine, valine, isoleucine and threonine. The quantity and presence of each amino acid depends on the doses and exposure of pesticides. Other amino acids were present in lower concentration and quantitative estimation was not possible. Total protein content showed a decreasing trend in all the pesticide exposed group from 5.64 g dl-1 to 2.65 g dl-1 as compared to control.    

 

 

 Key words

 

Amino acid, Clarias batrachus, Endocel, Rogor, Thin layer chromatography, Total protein

 

 

 

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