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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue May 2015, 36 (3)                                     Back


nstantaneous and historical temperature effects on a-pinene

Molecular identification of natural  sand fly species populations inferred from ITS2 rRNA gene in Saudi Arabia 

 

Reem A. Al-Ajmi1, Tahany H. Ayaad1,2*, Mashael Al-Anazi3, Mohammed N. Al-Ahdal3 and Ahmed A. Al-Qahtani3, 4

1Zoology Department, Faculty of Science, King Saud University, Riyadh, 2457,11451, Saudi Arabia

2Entomology Department,Faculty of Science  ,Cairo University,Giza,11613,Egypt

3Department of Infection and Immunity, Research Center, King Faisal Specialist Hospital and Research Center, Riyadh,3354, 11211, Saudi Arabia

4Liver Disease Research Center, King Saud University, Riyadh, 2925,11461, Saudi Arabia

*Corresponding Author?s E-mail: ayaadmtah@gmail.com

 

 

 

Publication Data

Paper received:

10 February 2014

 

Revised received:

19 September 2014

 

Re-revised received:

29 November 2014

 

Accepted:

08 December 2014

 

Abstract

Sandflies are blood-sucking insects of great medical importance and are transmission vectors for several organisms that are infectious to humans. Three hundred fourteen adult sandflies were collected and morphologically identified as five species: Phlebotomus papatasi, P. bergeroti, P. sergenti, Sergentomyia antennata and S. clydei. P. papatasi was the most abundant species (22.29%) of central region of Saudi Arabia, while S. clydei was the most abundant species (18.79 %) of southwestern region. Molecular classification of sandflies was done based on ribosomal RNA (rRNA) gene Internal Transcribed Spacer 2 (ITS2) sequences. ITS2 region was amplified using specific primers designed from a conserved region. Several clones from each individual were sequenced and analyzed using different DNA sequence analysis software. The results showed moderate variations (0.06%) between clones of same P. papatasi individual.  However, P. sergenti individuals were highly divergent (0.207%). A phylogenetic tree, based on neighbor-joining analysis, showed that morphologically identical individuals of same species exhibited significant variations at the molecular level. Cloned P. bergeroti sequences were highly related to P. papatasi sequences (0.05%). Further, S. antennata species sequences were similar to S. clydei species sequences. In conclusion, molecular typing of sand fly species from Saudi Arabia based on ITS2 region may aid in differentiating between species and, possibly, sub-species.    

 

 

 Key words

ITS2, Ribosomal RNA gene, Sandfly

 

 

 

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