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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue May 2015, 36 (3)                                     Back


nstantaneous and historical temperature effects on a-pinene

Study on combined effects of ochratoxin A and endosulfan on antioxidant enzymes in rats 

 

Shashi Nandar Kumar1, Avinash Gopal Telang2, Rajendra Damu Patil3, Karam Pal Singh2,

Arun Kumar Jain1 and Rinku Sharma4*

1Environmental Toxicology Laboratory, National Institute of Pathology (ICMR), New Delhi-110 029, India

2Centre for Animal Disease Research and Diagnosis (CADRAD), Indian Veterinary Research Institute, Bareilly-243 122, India

3Department of Veterinary Pathology, Dr. G.C. Negi College of Veterinary and Animal Sciences,

Himachal Pradesh Agricultural University, Palampur-176 062, India

4Disease Investigation Laboratory, Regional Station, Indian Veterinary Research Institute, Palampur-176 061, India

*Corresponding Author?s E-mail: rinkusharma99@gmail.com

 

 

 

Publication Data

Paper received:

05 March 2013

 

Revised received:

10 January 2014

 

Re-revised received:

18 March 2014

 

Accepted:

22 July 2014

 

Abstract

The aim of the present study was to determine the effects of ochratoxin A (OTA) and endosulfan, alone and in combination, on oxidative stress in rats. In total of 40 male Wistar rats were randomly divided into four groups of 10 rats each: Control (Standard diet); OTA (at 4ppm through diet); endosulfan (at 5mg kg-1 body weight in corn oil by oral intubation) and OTA plus endosulfan (at 4ppm OTA plus 5mg kg-1 body weight endosulfan). The level of lipid peroxidation (LPO), catalase, superoxide dismutase (SOD) and reduced glutathione (GSH) were measured in erythrocytes as well as in tissue homogenates of liver and kidneys, after 30 days post-intoxication. OTA and endosulfan treatment caused significant elevation in LPO levels in erythrocytes (62%), liver (28%) and kidneys (125%). However, the level of antioxidant enzymes such as catalase, SOD and GSH decreased (20% to 50%) in all the treated groups. It was concluded that oxidative stress was more severe in case of combination treatment, which might be due additive toxic effect of endosulfan and OTA in rats.   

 

 

 Key words

Antioxidant enzymes, Endosulfan, Lipid peroxidation, Ochratoxin A, Wistar rat

 

 

 

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